Detailed Information on Publication Record
2002
Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography
GLATZ, Zdeněk and Petra ŠEVČÍKOVÁBasic information
Original name
Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography
Authors
GLATZ, Zdeněk (203 Czech Republic, guarantor) and Petra ŠEVČÍKOVÁ (203 Czech Republic)
Edition
Journal of Chromatography B, Amstredam, Elsevier Science, 2002, 0378-4347
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.911 in 2001
RIV identification code
RIV/00216224:14310/02:00005543
Organization unit
Faculty of Science
UT WoS
000175169900027
Keywords in English
DTT; MEKC; on-column;
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems.
Links
| GA525/00/0785, research and development project |
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