Immunohistochemical study of the extracellular matrix formed
during peripheral nerve regeneration through a knitted
prosthesis

J 2001

Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis

SVÍŽENSKÁ, Ivana and Petr DUBOVÝ

Basic information

Original name

Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis

Name in Czech

Imunohistochemická studie ECM během regenerace periferního nervu přes pletenou protézu

Authors

SVÍŽENSKÁ, Ivana (203 Czech Republic) and Petr DUBOVÝ (203 Czech Republic, guarantor)

Edition

Scripta medica, Brno, Masarykova univerzita, Lékařská fakulta, 2001, 1211-3395

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/01:00005656

Organization unit

Faculty of Medicine

Keywords in English

Regeneration; Peripheral nerve; Extracellular matrix; Immunohistochemistry

Abstract

ORIG CZ

V originále

The extracellular matrix formed in a knitted prosthesis, which was applied between the stumps of a transected sciatic nerve of the rat, was investigated to find out whether it provided suitable conditions for nerve regeneration. After 6 weeks, the prosthesis contained a nerve outgrowth connecting the nerve stumps. The immunohistochemical staining of the cryostat sections through the middle of the nerve prosthesis revealed that thrombospondin, fibronectin, chondroitin sulfate A and C, and type IV collagen were localised in the connective tissue surrounding the nerve outgrowth, in the perineurium of individual minifascicles, as well as in the endoneurium among regenerated nerve fibres. No immunostaining for vitronectin was observed in cross-sections through the nerve outgrowth. Our results suggest that the environment formed during nerve regrowth within a knitted prosthesis provides conditions supporting the regeneration of nerve fibres.

In Czech

Extracelulární matrix je tvořena podél regenerujících axonů v pletené nervové protéze.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

Citovat

SVÍŽENSKÁ, Ivana and Petr DUBOVÝ. Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis. Scripta medica. Brno: Masarykova univerzita, Lékařská fakulta, 2001, vol. 74, No 3, p. 221-230. ISSN 1211-3395.

@article{403231,
   author = {Svíženská, Ivana and Dubový, Petr},
   article_location = {Brno},
   article_number = {3},
   keywords = {Regeneration; Peripheral nerve; Extracellular matrix; Immunohistochemistry},
   language = {eng},
   issn = {1211-3395},
   journal = {Scripta medica},
   title = {Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis},
   volume = {74},
   year = {2001}
}

TY  - JOUR
ID  - 403231
AU  - Svíženská, Ivana - Dubový, Petr
PY  - 2001
TI  - Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis
JF  - Scripta medica
VL  - 74
IS  - 3
SP  - 221
EP  - 221
PB  - Masarykova univerzita, Lékařská fakulta
SN  - 12113395
KW  - Regeneration
KW  - Peripheral nerve
KW  - Extracellular matrix
KW  - Immunohistochemistry
N2  - The extracellular matrix formed in a knitted prosthesis, which was applied between the stumps of a transected sciatic nerve of the rat, was investigated to find out whether it provided suitable conditions for nerve regeneration. After 6 weeks, the prosthesis contained a nerve outgrowth connecting the nerve stumps. The immunohistochemical staining of the cryostat sections through the middle of the nerve prosthesis revealed that thrombospondin, fibronectin, chondroitin sulfate A and C, and type IV collagen were localised in the connective tissue surrounding the nerve outgrowth, in the perineurium of individual minifascicles, as well as in the endoneurium among regenerated nerve fibres. No immunostaining for vitronectin was observed in cross-sections through the nerve outgrowth. Our results suggest that the environment formed during nerve regrowth within a knitted prosthesis provides conditions supporting the regeneration of nerve fibres.
ER  -

SVÍŽENSKÁ, Ivana and Petr DUBOVÝ. Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis. \textit{Scripta medica}. Brno: Masarykova univerzita, Lékařská fakulta, 2001, vol.~74, No~3, p.~221-230. ISSN~1211-3395.

Detailed Information on Publication Record