Characterization of yellow-pigmented and motile enterococci
isolated from intestines of the garden snail Helix aspersa

J 2002

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT et. al.

Základní údaje

Originální název

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa

Autoři

ŠVEC, Pavel (203 Česká republika, garant), Luc DEVRIESE (56 Belgie), Ivo SEDLÁČEK (203 Česká republika), Margo BAELE (56 Belgie), Marc VANCANNEYT (56 Belgie), Freddy HAESEBROUCK (56 Belgie), Jean SWINGS (56 Belgie) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Journal of Applied Microbiology, Oxford, UK, Blackwell Science Ltd, 2002, 1365-2672

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

[Abstract] [Full text]

Impakt faktor

Impact factor: 1.819

Kód RIV

RIV/00216224:14310/02:00006086

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000175184500020

Klíčová slova anglicky

Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA

Štítky

16S Ribosomal RNA, Bacterial Typing Techniques, CLASSIFICATION, DNA, Enterococcus, Helix (Snails), Nucleic Acid Hybridization, Pigments/metabolism

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 6. 2009 12:16, doc. RNDr. Pavel Švec, Ph.D.

Anotace

V originále

AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.

Návaznosti

GA301/02/1505, projekt VaV

Název: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Grantová agentura ČR, Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Citovat

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT, Freddy HAESEBROUCK, Jean SWINGS a Jiří DOŠKAŘ. Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa. Journal of Applied Microbiology. Oxford, UK: Blackwell Science Ltd, 2002, roč. 92, č. 5, s. 951-957. ISSN 1365-2672.

@article{403808,
   author = {Švec, Pavel and Devriese, Luc and Sedláček, Ivo and Baele, Margo and Vancanneyt, Marc and Haesebrouck, Freddy and Swings, Jean and Doškař, Jiří},
   article_location = {Oxford, UK},
   article_number = {5},
   keywords = {Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA},
   language = {eng},
   issn = {1365-2672},
   journal = {Journal of Applied Microbiology},
   title = {Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa},
   url = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2002.01605.x},
   volume = {92},
   year = {2002}
}

TY  - JOUR
ID  - 403808
AU  - Švec, Pavel - Devriese, Luc - Sedláček, Ivo - Baele, Margo - Vancanneyt, Marc - Haesebrouck, Freddy - Swings, Jean - Doškař, Jiří
PY  - 2002
TI  - Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail Helix aspersa
JF  - Journal of Applied Microbiology
VL  - 92
IS  - 5
SP  - 951
EP  - 951
PB  - Blackwell Science Ltd
SN  - 13652672
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Enterococcus
KW  - classification;Helix (Snails)
KW  - Nucleic Acid Hybridization
KW  - Pigments/metabolism;16S Ribosomal RNA
UR  - http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2672.2002.01605.x
N2  - AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.
ER  -

ŠVEC, Pavel, Luc DEVRIESE, Ivo SEDLÁČEK, Margo BAELE, Marc VANCANNEYT, Freddy HAESEBROUCK, Jean SWINGS a Jiří DOŠKAŘ. Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail $<$I$>$Helix aspersa$<$/I$>$. \textit{Journal of Applied Microbiology}. Oxford, UK: Blackwell Science Ltd, 2002, roč.~92, č.~5, s.~951-957. ISSN~1365-2672.

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