TRNKOVÁ, Libuše, René KIZEK a Jan VACEK. Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping. Bioelectrochemistry. The Netherlands: Elsevier, 2002, roč. 3, č. 56, s. 57-61. ISSN 1567-5394. |
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@article{403896, author = {Trnková, Libuše and Kizek, René and Vacek, Jan}, article_location = {The Netherlands}, article_number = {56}, keywords = {Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration}, language = {eng}, issn = {1567-5394}, journal = {Bioelectrochemistry}, title = {Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping}, volume = {3}, year = {2002} }
TY - JOUR ID - 403896 AU - Trnková, Libuše - Kizek, René - Vacek, Jan PY - 2002 TI - Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping JF - Bioelectrochemistry VL - 3 IS - 56 SP - 57 EP - 57 PB - Elsevier SN - 15675394 KW - Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration N2 - Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V ), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research. ER -
TRNKOVÁ, Libuše, René KIZEK a Jan VACEK. Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2002, roč.~3, č.~56, s.~57-61. ISSN~1567-5394.
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