Automated micro axial tomography of cell nuclei after specific
labelling by fluorescence in situ hybridisation

J 2002

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH et. al.

Základní údaje

Originální název

Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation

Autoři

KOZUBEK, Michal (203 Česká republika, garant), Magdalena SKALNÍKOVÁ (203 Česká republika), Petr MATULA (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Joachim RAUCH (276 Německo), Friedrich NEUHAUS (276 Německo), Heinz EIPEL (276 Německo) a Michael HAUSMANN (276 Německo)

Vydání

Micron, Amsterdam, Elsevier, 2002, 0968-4328

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.537

Kód RIV

RIV/00216224:14330/02:00006215

Organizační jednotka

Fakulta informatiky

Klíčová slova anglicky

micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei

Štítky

automated microscopy, cbia-web, FISH imaging, high-resolution cytometry, interphase cell nuclei, micro axial tomography

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 5. 2009 10:59, doc. RNDr. Petr Matula, Ph.D.

Anotace

V originále

Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre.

Návaznosti

MSM 143300002, záměr

Název: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii

Citovat

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH, Friedrich NEUHAUS, Heinz EIPEL a Michael HAUSMANN. Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation. Micron. Amsterdam: Elsevier, 2002, roč. 33, č. 7, s. 655-665. ISSN 0968-4328.

@article{404450,
   author = {Kozubek, Michal and Skalníková, Magdalena and Matula, Petr and Bártová, Eva and Rauch, Joachim and Neuhaus, Friedrich and Eipel, Heinz and Hausmann, Michael},
   article_location = {Amsterdam},
   article_number = {7},
   keywords = {micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei},
   language = {eng},
   issn = {0968-4328},
   journal = {Micron},
   title = {Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation},
   volume = {33},
   year = {2002}
}

TY  - JOUR
ID  - 404450
AU  - Kozubek, Michal - Skalníková, Magdalena - Matula, Petr - Bártová, Eva - Rauch, Joachim - Neuhaus, Friedrich - Eipel, Heinz - Hausmann, Michael
PY  - 2002
TI  - Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation
JF  - Micron
VL  - 33
IS  - 7
SP  - 655
EP  - 655
PB  - Elsevier
SN  - 09684328
KW  - micro axial tomography
KW  - automated microscopy
KW  - high-resolution cytometry
KW  - FISH imaging
KW  - interphase cell nuclei
N2  - Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre.
ER  -

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Joachim RAUCH, Friedrich NEUHAUS, Heinz EIPEL a Michael HAUSMANN. Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation. \textit{Micron}. Amsterdam: Elsevier, 2002, roč.~33, č.~7, s.~655-665. ISSN~0968-4328.

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