Development of software for automated micro-axialtomography

D 2002

Development of software for automated micro-axialtomography

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL et. al.

Základní údaje

Originální název

Development of software for automated micro-axialtomography

Autoři

KOZUBEK, Michal (203 Česká republika, garant), Magdalena SKALNÍKOVÁ (203 Česká republika), Petr MATULA (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Heinz EIPEL (276 Německo) a Michael HAUSMANN (276 Německo)

Vydání

Brno, Biophysics of the genome and its interactions, s. 45-53, 2002

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14330/02:00006218

Organizační jednotka

Fakulta informatiky

ISBN

80-210-2853-X

Klíčová slova anglicky

micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei

Štítky

automated microscopy, FISH imaging, high-resolution cytometry, interphase cell nuclei, micro axial tomography

Změněno: 10. 7. 2002 11:20, prof. RNDr. Michal Kozubek, Ph.D.

Anotace

V originále

Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can be observed in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the description of the algorithms for image acquisition and analysis.

Návaznosti

MSM 143300002, záměr

Název: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii

Citovat

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL a Michael HAUSMANN. Development of software for automated micro-axialtomography. In Biophysics of the genome and its interactions. Brno: Masaryk University, 2002, s. 45-53. ISBN 80-210-2853-X.

@inproceedings{404454,
   author = {Kozubek, Michal and Skalníková, Magdalena and Matula, Petr and Bártová, Eva and Eipel, Heinz and Hausmann, Michael},
   address = {Brno},
   booktitle = {Biophysics of the genome and its interactions},
   keywords = {micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2853-X},
   pages = {45-53},
   publisher = {Masaryk University},
   title = {Development of software for automated micro-axialtomography},
   year = {2002}
}

TY  - JOUR
ID  - 404454
AU  - Kozubek, Michal - Skalníková, Magdalena - Matula, Petr - Bártová, Eva - Eipel, Heinz - Hausmann, Michael
PY  - 2002
TI  - Development of software for automated micro-axialtomography
PB  - Masaryk University
CY  - Brno
SN  - 802102853X
KW  - micro axial tomography
KW  - automated microscopy
KW  - high-resolution cytometry
KW  - FISH imaging
KW  - interphase cell nuclei
N2  - Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can be observed in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the description of the algorithms for image acquisition and analysis.
ER  -

KOZUBEK, Michal, Magdalena SKALNÍKOVÁ, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL a Michael HAUSMANN. Development of software for automated micro-axialtomography. In \textit{Biophysics of the genome and its interactions}. Brno: Masaryk University, 2002, s.~45-53. ISBN~80-210-2853-X.

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