Monitornig of Cysteine in Human Urine by On-column
Derivatization during MEKC

D 2002

Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ

Základní údaje

Originální název

Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC

Autoři

ŠEVČÍKOVÁ, Petra (203 Česká republika) a Zdeněk GLATZ (203 Česká republika, garant)

Vydání

2002. vyd. Olomouc, Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002, s. 84-84, 2002

Nakladatel

Česká společnost chemická - skupina pro chromatografii a elektroforézu

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006230

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-86238-24-5

Klíčová slova anglicky

derivatization; on-column; urine; MEKC

Štítky

derivatization, MEKC, on-column, urine

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Cysteine is a high sulphur containing amino acid synthesised by the liver. It is involved in a variety of important cellular functions, among others protein synthesis, detoxication and metabolisms. The biological significance of cysteine is well established but it is only recently that its level in physiological fluids such as plasma and urine has been established as an important indicator for a number of clinical disorders. Determination of urinary content of cysteine consequently may be taken as helpful non-invasive methods of diagnosis. This paper describes the development and validation of a micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide method for the analysis of free and total cysteine in human urine. 2,2-dipyridyldisulphide and a sample of cysteine are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and cysteine, on-column mixing and reaction occur. Cysteine is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.98) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 2.18 % and the inter-day reproducibility of the migration time 0.51 %.

Návaznosti

GA525/00/0785, projekt VaV

Název: Acidifikační procesy v sulfidových odpadech

Investor: Grantová agentura ČR, Acidifikační procesy v sulfidových odpadech

Citovat

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC. In Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002. 2002. vyd. Olomouc: Česká společnost chemická - skupina pro chromatografii a elektroforézu, 2002, s. 84. ISBN 80-86238-24-5.

@inproceedings{404488,
   author = {Ševčíková, Petra and Glatz, Zdeněk},
   address = {Olomouc},
   booktitle = {Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002},
   edition = {2002},
   keywords = {derivatization; on-column; urine; MEKC},
   language = {eng},
   location = {Olomouc},
   isbn = {80-86238-24-5},
   pages = {84-84},
   publisher = {Česká společnost chemická - skupina pro chromatografii a elektroforézu},
   title = {Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC},
   year = {2002}
}

TY  - JOUR
ID  - 404488
AU  - Ševčíková, Petra - Glatz, Zdeněk
PY  - 2002
TI  - Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC
PB  - Česká společnost chemická - skupina pro chromatografii a elektroforézu
CY  - Olomouc
SN  - 8086238245
KW  - derivatization
KW  - on-column
KW  - urine
KW  - MEKC
N2  - Cysteine is a high sulphur containing amino acid synthesised by the liver. It is involved in a variety of important cellular functions, among others protein synthesis, detoxication and metabolisms. The biological significance of cysteine is well established but it is only recently that its level in physiological fluids such as plasma and urine has been established as an important indicator for a number of clinical disorders. Determination of urinary content of cysteine consequently may be taken as helpful non-invasive methods of diagnosis. This paper describes the development and validation of a micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide method for the analysis of free and total cysteine in human urine. 2,2-dipyridyldisulphide and a sample of cysteine are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and cysteine, on-column mixing and reaction occur. Cysteine is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.98) with a detection limit of 2.5 mM. The inter-day reproducibility of the peak area was 2.18 % and the inter-day reproducibility of the migration time 0.51 %.
ER  -

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Monitornig of Cysteine in Human Urine by On-column Derivatization during MEKC. In \textit{Book of abstracts of Developments in Chromatography and Electrophoresis \&{} Chiranal 2002}. 2002. vyd. Olomouc: Česká společnost chemická - skupina pro chromatografii a elektroforézu, 2002, s.~84. ISBN~80-86238-24-5.

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