Analysis of Lignans in Schisandra Extract, Comparison between
CECand MEKC

D 2002

Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE et. al.

Základní údaje

Originální název

Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC

Autoři

KVASNIČKOVÁ, Leona (203 Česká republika), Hana ŠTĚRBOVÁ (203 Česká republika), Petra ŠEVČÍKOVÁ (203 Česká republika), Zdeněk GLATZ (203 Česká republika, garant), Vladislav KAHLE (203 Česká republika) a Jiří SLANINA (203 Česká republika)

Vydání

2002. vyd. Olomouc, Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002, s. 74-74, 2002

Nakladatel

Česká společnost chemická - skupina pro chromatografii a elektroforézu

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006232

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-86238-24-5

Klíčová slova anglicky

Lignans; Schisandra; CEC; MEKC

Štítky

CEC, lignans, MEKC, Schisandra

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Schisandra chinensis BAILL. (Schisandraceae/Magnoliaceae) is a famous medicinal plant native to East Asia. It grows wild mainly in northeastern China, Korea and Japan and also in the most Eastern parts of Russia. The biologically active compounds are the lignans with an uncommon structure derived from dibenzo[a,c]cyclooctadiene. The lignans prevent liver injuries stimulate liver regeneration and also inhibit hepatocarcinogenesis and lipid peroxidation. Moreover several reports indicate, that the lignans have effects on human intellectual activity and physical performance. Recently, some dibenzo[a,c]cyclooctadiene lignans were identified as potent anti-HIV agent. Several analytical methods have been reported for the determinations of the lignans in Schisandra plant. Previously described methods include thin layer and high-performance liquid chromatographic techniques. Gas and high-performance liquid chromatography in combination with mass spectroscopy have also been demonstrated to be effective analytical method for the determination of the lignans in various samples. In this study we report capillary electrochromatography (CEC) in macroporous polyacrylamide columns and micellar electrokinetic capillary chromatography (MEKC) for the determination of several lignans - schizandrin, gomisin A, gomisin N and wuweizisu C, the major compounds of the Schisandra extracts. The reproducibilities of peak areas and migration times and linearities and limits of detection were investigated and compared. The results of quantitative analyses were compared as well, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by MEKC and with previous literature data.

Návaznosti

GA203/02/1447, projekt VaV

Název: Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Investor: Grantová agentura ČR, Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Citovat

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE a Jiří SLANINA. Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC. In Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002. 2002. vyd. Olomouc: Česká společnost chemická - skupina pro chromatografii a elektroforézu, 2002, s. 74. ISBN 80-86238-24-5.

@inproceedings{404490,
   author = {Kvasničková, Leona and Štěrbová, Hana and Ševčíková, Petra and Glatz, Zdeněk and Kahle, Vladislav and Slanina, Jiří},
   address = {Olomouc},
   booktitle = {Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002},
   edition = {2002},
   keywords = {Lignans; Schisandra; CEC; MEKC},
   language = {eng},
   location = {Olomouc},
   isbn = {80-86238-24-5},
   pages = {74-74},
   publisher = {Česká společnost chemická - skupina pro chromatografii a elektroforézu},
   title = {Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC},
   year = {2002}
}

TY  - JOUR
ID  - 404490
AU  - Kvasničková, Leona - Štěrbová, Hana - Ševčíková, Petra - Glatz, Zdeněk - Kahle, Vladislav - Slanina, Jiří
PY  - 2002
TI  - Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC
PB  - Česká společnost chemická - skupina pro chromatografii a elektroforézu
CY  - Olomouc
SN  - 8086238245
KW  - Lignans
KW  - Schisandra
KW  - CEC
KW  - MEKC
N2  - Schisandra chinensis BAILL. (Schisandraceae/Magnoliaceae) is a famous medicinal plant native to East Asia. It grows wild mainly in northeastern China, Korea and Japan and also in the most Eastern parts of Russia. The biologically active compounds are the lignans with an uncommon structure derived from dibenzo[a,c]cyclooctadiene. The lignans prevent liver injuries stimulate liver regeneration and also inhibit hepatocarcinogenesis and lipid peroxidation. Moreover several reports indicate, that the lignans have effects on human intellectual activity and physical performance. Recently, some dibenzo[a,c]cyclooctadiene lignans were identified as potent anti-HIV agent. Several analytical methods have been reported for the determinations of the lignans in Schisandra plant. Previously described methods include thin layer and high-performance liquid chromatographic techniques. Gas and high-performance liquid chromatography in combination with mass spectroscopy have also been demonstrated to be effective analytical method for the determination of the lignans in various samples. In this study we report capillary electrochromatography (CEC) in macroporous polyacrylamide columns and micellar electrokinetic capillary chromatography (MEKC) for the determination of several lignans - schizandrin, gomisin A, gomisin N and wuweizisu C, the major compounds of the Schisandra extracts. The reproducibilities of peak areas and migration times and linearities and limits of detection were investigated and compared. The results of quantitative analyses were compared as well, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by MEKC and with previous literature data.
ER  -

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Petra ŠEVČÍKOVÁ, Zdeněk GLATZ, Vladislav KAHLE a Jiří SLANINA. Analysis of Lignans in Schisandra Extract, Comparison between CECand MEKC. In \textit{Book of abstracts of Developments in Chromatography and Electrophoresis \&{} Chiranal 2002}. 2002. vyd. Olomouc: Česká společnost chemická - skupina pro chromatografii a elektroforézu, 2002, s.~74. ISBN~80-86238-24-5.

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