Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes

AMRICHOVÁ, Jana, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK, Pavla JIRSOVÁ and Michal KOZUBEK. Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes. In Biophysics of the genome and its interactions. Brno: Masaryk University, 2002, p. 1-6. ISBN 80-210-2853-X.

Basic information

• Original name: Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes
• Authors: AMRICHOVÁ, Jana (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, guarantor).
• Edition: Brno, Biophysics of the genome and its interactions, p. 1-6, 2002.
• Publisher: Masaryk University

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14330/02:00006233
• Organization unit: Faculty of Informatics
• ISBN: 80-210-2853-X
• Keywords in English: telomere; centromere; interpahse nuclei; chromatin structure
• Tags: centromere, chromatin structure, interpahse nuclei, telomere

Abstract

Nuclear location of subtelomeric and centromeric DNA sequences of chromosome 8, 9 and 13 was investigated in 3D fixed human G0 and stimulated T-lymphocytes using repeated dual colour FISH. In the first hybridisation, DNA probes for p and q arms labelled by different fluorochromes were used. The centromere and the chromosome territory of the same chromosome were visualised in the second hybridisation on the same nuclei. It was found for all investigated chromosomes that contrary to centromeres located in the proximity of the nuclear membrane, the telomeres adopt more central position. These results indicate a hairpin like structure of chromosomes in the interphase nucleus. The results of this study provide information not only on the topography of subtelomeres but also images of the spatial arrangement and orientation of interphase chromosomes in cycling and non-cycling cell nuclei.

Links

• GA202/01/0197, research and development project: Name: Ionizující záření jako nástroj pro výzkum struktury buněčného jádra a pro vývoj nových technik pro ekologii a medicínu

Investor: Czech Science Foundation, Ionising radiation as a tool in the investigations of the cell nuclear structure and in the development of new techniques for ecology and medicine

• GA301/01/0186, research and development project: Name: Studium lokální kontroly exprese genů pomocí spektrální mikroskopie a analýzy obrazu

Investor: Czech Science Foundation, Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques

• IAA1065203, research and development project: Name: Využití kombinace laserových mikrosvazkových a cytometrických technik ke studiu struktury a dynamiky lidského genomu

Investor: Academy of Sciences of the Czech Republic, The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome

• MSM 143300002, plan (intention): Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

• NC5955, research and development project: Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů.

Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.