A quantitative immunohistochemical study of the endoneurium in
the rat dorsal and ventral spinal roots

J 2002

A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ

Základní údaje

Originální název

A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots

Název česky

Kvantitativní imunohistochemické studium endoneuria dorzálních a ventrálních kořenů spinálních nervů

Autoři

DUBOVÝ, Petr (203 Česká republika, garant), Ilona KLUSÁKOVÁ (203 Česká republika) a Ivana SVÍŽENSKÁ (203 Česká republika)

Vydání

Histochemistry and Cell Biology, Heidelberg, Springer, 2002, 0948-6143

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.054

Kód RIV

RIV/00216224:14110/02:00006287

Organizační jednotka

Lékařská fakulta

UT WoS

000176933000002

Klíčová slova anglicky

extracellular; matrix; regeneration

Štítky

extracellular, matrix, Regeneration

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 6. 2009 10:45, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.

Česky

Endoneuriální ECM podél nervových vláken ve dorzálních a ventrálních kořenech spinálních nervů mají rozdílné molekulární složení.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. Histochemistry and Cell Biology. Heidelberg: Springer, 2002, roč. 117, č. 6, s. 473-480. ISSN 0948-6143.

@article{404708,
   author = {Dubový, Petr and Klusáková, Ilona and Svíženská, Ivana},
   article_location = {Heidelberg},
   article_number = {6},
   keywords = {extracellular; matrix; regeneration},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots},
   volume = {117},
   year = {2002}
}

TY  - JOUR
ID  - 404708
AU  - Dubový, Petr - Klusáková, Ilona - Svíženská, Ivana
PY  - 2002
TI  - A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots
JF  - Histochemistry and Cell Biology
VL  - 117
IS  - 6
SP  - 473
EP  - 473
PB  - Springer
SN  - 09486143
KW  - extracellular
KW  - matrix
KW  - regeneration
N2  - The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2002, roč.~117, č.~6, s.~473-480. ISSN~0948-6143.

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