A quantitative immunohistochemical study of the endoneurium in
the rat dorsal and ventral spinal roots

J 2002

A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots

DUBOVÝ, Petr; Ilona KLUSÁKOVÁ and Ivana SVÍŽENSKÁ

Basic information

Original name

A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots

Name in Czech

Kvantitativní imunohistochemické studium endoneuria dorzálních a ventrálních kořenů spinálních nervů

Authors

DUBOVÝ, Petr; Ilona KLUSÁKOVÁ and Ivana SVÍŽENSKÁ

Edition

Histochemistry and Cell Biology, Heidelberg, Springer, 2002, 0948-6143

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.054

RIV identification code

RIV/00216224:14110/02:00006287

Organization unit

Faculty of Medicine

UT WoS

000176933000002

Keywords in English

extracellular; matrix; regeneration

Abstract

ORIG CZ

In the original language

The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.

In Czech

Endoneuriální ECM podél nervových vláken ve dorzálních a ventrálních kořenech spinálních nervů mají rozdílné molekulární složení.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

Cite

DUBOVÝ, Petr; Ilona KLUSÁKOVÁ and Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. Histochemistry and Cell Biology. Heidelberg: Springer, 2002, vol. 117, No 6, p. 473-480. ISSN 0948-6143.

@article{404708,
   author = {Dubový, Petr and Klusáková, Ilona and Svíženská, Ivana},
   article_location = {Heidelberg},
   article_number = {6},
   keywords = {extracellular; matrix; regeneration},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots},
   volume = {117},
   year = {2002}
}

TY  - JOUR
ID  - 404708
AU  - Dubový, Petr - Klusáková, Ilona - Svíženská, Ivana
PY  - 2002
TI  - A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots
JF  - Histochemistry and Cell Biology
VL  - 117
IS  - 6
SP  - 473
EP  - 473
PB  - Springer
SN  - 09486143
KW  - extracellular
KW  - matrix
KW  - regeneration
N2  - The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM) among them is produced by Schwann cells and fibroblasts under the control of the axons. Chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin are common components of the endoneurial ECM involved in the normal function as well as regeneration of the peripheral nerve. The present paper demonstrates a comparison of immunofluorescence staining for chondroitin sulfate proteoglycan, fibronectin, tenascin-C, and thrombospondin in the endoneurium of the rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were cut simultaneously and adhered to the same microscopic slide. They were incubated simultaneously and the intensity of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized pictures to select the areas of measurement. The measurement of the immunofluorescence brightness revealed that the endoneurium of the dorsal roots was immunostained for the studied molecules at a higher intensity than in the ventral roots. The results suggest quantitative differences of the endoneurial content of the spinal dorsal and ventral roots probably corresponding to the presence of various types of axons. On the other hand, the different concentration of ECM molecules in the endoneurium of dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
ER  -

DUBOVÝ, Petr; Ilona KLUSÁKOVÁ and Ivana SVÍŽENSKÁ. A quantitative immunohistochemical study of the endoneurium in the rat dorsal and ventral spinal roots. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2002, vol.~117, No~6, p.~473-480. ISSN~0948-6143.

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