Determination of Selenium in Blood Serum by Inductively Coupled
Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

J 2002

Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

MACHÁT, Jiří, Viktor KANICKÝ a Vítězslav OTRUBA

Základní údaje

Originální název

Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

Autoři

MACHÁT, Jiří (203 Česká republika), Viktor KANICKÝ (203 Česká republika) a Vítězslav OTRUBA (203 Česká republika, garant)

Vydání

Analytical and Bioanalytical Chemistry, Heidelberg, Springer, 2002, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006344

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000176103100012

Klíčová slova anglicky

Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization

Štítky

ICP-AES, Pneumatic nebulization, Selenium speciation, Spectrometry

Změněno: 20. 5. 2003 14:53, Mgr. Jiří Machát, Ph.D.

Anotace

V originále

The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181.

Návaznosti

MSM 143100003, záměr

Název: Studium plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Studium plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Citovat

MACHÁT, Jiří, Viktor KANICKÝ a Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. Analytical and Bioanalytical Chemistry. Heidelberg: Springer, 2002, roč. 372, č. 4, s. 576-581. ISSN 1618-2642.

@article{405042,
   author = {Machát, Jiří and Kanický, Viktor and Otruba, Vítězslav},
   article_location = {Heidelberg},
   article_number = {4},
   keywords = {Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical Chemistry},
   title = {Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization},
   volume = {372},
   year = {2002}
}

TY  - JOUR
ID  - 405042
AU  - Machát, Jiří - Kanický, Viktor - Otruba, Vítězslav
PY  - 2002
TI  - Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization
JF  - Analytical and Bioanalytical Chemistry
VL  - 372
IS  - 4
SP  - 576
EP  - 576
PB  - Springer
SN  - 16182642
KW  - Selenium speciation
KW  - Spectrometry
KW  - ICP-AES
KW  - Pneumatic nebulization
N2  - The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181.
ER  -

MACHÁT, Jiří, Viktor KANICKÝ a Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: Springer, 2002, roč.~372, č.~4, s.~576-581. ISSN~1618-2642.

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