Degradation of 4-nitroguaiacol and other nitroaromatic
compounds by bacterial strain J7

D 2002

Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC, Eva DURNOVÁ, Ivo SEDLÁČEK et. al.

Basic information

Original name

Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7

Authors

NAVRÁTILOVÁ, Jitka (203 Czech Republic, guarantor), Ludmila KOTOUČKOVÁ (203 Czech Republic), Miroslav NĚMEC (203 Czech Republic), Eva DURNOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

Brno, VI. Pracovní setkání biochemiků a molekulárních biologů, p. 71-71, 2002

Publisher

Masarykova univerzita v Brně

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00006347

Organization unit

Faculty of Science

ISBN

80-210-2777-0

Keywords in English

degradation;nitroaromatic compounds; Rhodococcus

Abstract

V originále

Bacterial strain J7 was isolated from soil. This bacterium is capable to degrade nitroaromatic compounds. The strain J7 is Gram positive, strict aerobic bacterium, who exhibit a typical irregular rod-coccus cycle with fragmentation. The strain J7 formed opaque, slightly convex, creamy colonies. The bacterium was isolated by selectively enrichment with 4-nitroguaiacol in mineral SBM medium. The optimum for growth and degradation of the strain activity is 28 stupňů Celsia. The strain J7 was identified as a member of the genus Rhodococcus on the basis of morfology, physiology, biochemical characterisation, whole cell fatty acid composition. and determination of partial sequence of genes for 16S rRNA. We suppose, that the strain J7 belongs to Rhodococcus opacus or Rhodococcus percolatus on the base of identity of 16S rRNA sequence. Degradation was tested for these nitroaromatic compounds: 4-nitrocatechol, 4-nitroguaiacol, 5-nitroguaiacol, 2-nitrophenol, 3-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-diphluoro-6-nitrophenol.

Links

GA204/00/P095, research and development project

Name: Mikrobiální transformace organických polutantů ve směsích

Investor: Czech Science Foundation, Microbial transformation of organic pollutants in mixtures

Citovat

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC, Eva DURNOVÁ and Ivo SEDLÁČEK. Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7. In VI. Pracovní setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita v Brně, 2002, p. 71. ISBN 80-210-2777-0.

@inproceedings{405059,
   author = {Navrátilová, Jitka and Kotoučková, Ludmila and Němec, Miroslav and Durnová, Eva and Sedláček, Ivo},
   address = {Brno},
   booktitle = {VI. Pracovní setkání biochemiků a molekulárních biologů},
   keywords = {degradation;nitroaromatic compounds; Rhodococcus},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2777-0},
   pages = {71-71},
   publisher = {Masarykova univerzita v Brně},
   title = {Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7},
   year = {2002}
}

TY  - JOUR
ID  - 405059
AU  - Navrátilová, Jitka - Kotoučková, Ludmila - Němec, Miroslav - Durnová, Eva - Sedláček, Ivo
PY  - 2002
TI  - Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7
PB  - Masarykova univerzita v Brně
CY  - Brno
SN  - 8021027770
KW  - degradation;nitroaromatic compounds
KW  - Rhodococcus
N2  - Bacterial strain J7 was isolated from soil. This bacterium is capable to degrade nitroaromatic compounds. The strain J7 is Gram positive, strict aerobic bacterium, who exhibit a typical irregular rod-coccus cycle with fragmentation. The strain J7 formed opaque, slightly convex, creamy colonies. The bacterium was isolated by selectively enrichment with 4-nitroguaiacol in mineral SBM medium. The optimum for growth and degradation of the strain activity is 28 stupňů Celsia. The strain J7 was identified as a member of the genus Rhodococcus on the basis of morfology, physiology, biochemical characterisation, whole cell fatty acid composition. and determination of partial sequence of genes for 16S rRNA. We suppose, that the strain J7 belongs to Rhodococcus opacus or Rhodococcus percolatus on the base of identity of 16S rRNA sequence. Degradation was tested for these nitroaromatic compounds: 4-nitrocatechol, 4-nitroguaiacol, 5-nitroguaiacol, 2-nitrophenol, 3-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-diphluoro-6-nitrophenol.
ER  -

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC, Eva DURNOVÁ and Ivo SEDLÁČEK. Degradation of 4-nitroguaiacol and other nitroaromatic compounds by bacterial strain J7. In \textit{VI. Pracovní setkání biochemiků a molekulárních biologů}. Brno: Masarykova univerzita v Brně, 2002, p.~71. ISBN~80-210-2777-0.

Detailed Information on Publication Record