Rhodococcus opacus J3 - the bacterium degrading nitroaromatic
compounds

D 2002

Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC and Jiří NEČA

Basic information

Original name

Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds

Authors

NAVRÁTILOVÁ, Jitka (203 Czech Republic, guarantor), Ludmila KOTOUČKOVÁ (203 Czech Republic), Miroslav NĚMEC (203 Czech Republic) and Jiří NEČA (203 Czech Republic)

Edition

Praha, 12th internationl biodeterioration and biodegradation symposium (bisosorption and biodremediaton III ), p. 56-56, Programme, Abstract book, 2002

Publisher

JMP Tisk s.r.o.

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00006348

Organization unit

Faculty of Science

ISBN

80-86313-08-5

Keywords in English

Rhodococcus opacus; nitroaromatic compounds; degradation

Abstract

V originále

Nitroaromatic compounds are released into the environment trough their wide use as dyes, plasticizers, pesticides, explozives and solvents. The presence of the nitro group causes such compounds to be more resistant to biodegradation than the unsubstituted analogs.The rhodococci have attracted and increased interest in the biochemical and genetical characterization of their metabolic capabilities, based on their ability to transform or degrade diverse classes of mainly hydrophobic substances. Rhodococcus opacus J3 is capable to degrade nitroaromatic compounds.This strain was isolated from soil by selectively enrichment with 4-nitrocatechol in mineral medium. Rhodococcus opacus J3 is gram-positive, strict aerobic bacterium who exhibit a typical rod-coccus cycle.This bacterium was identified as Rhodococcus opacus on the base of morfology, physiology, biochemical characterization, whole cell fatty acid composition and determination of partial sequence of genes for 16S rRNA. Biodegradation of 9 nitroaromatics was tested.We determined degradation of 5 substrates:4-nitrocatechol, 4-nitroguaiacol, 5-nitroguaiacol, 3-nitrophenol and 2,4-dinitrobenzoic acid. We tested degradation in concentration range from 0,025 mM to 0,1 mM. We did not determine degradation of 2-nitrophenol, 4-nitrophenol, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-diphluoro-6- nitrophenol. Concentration of nitroaromatics and nitrites released were measured spectrophotometricaly. Degradation of substrates was confirmed by HPLC. The process of degradation was monitored on automatic microbiological system Bioscreen C.

Links

GA204/00/P095, research and development project

Name: Mikrobiální transformace organických polutantů ve směsích

Investor: Czech Science Foundation, Microbial transformation of organic pollutants in mixtures

Citovat

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC and Jiří NEČA. Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds. In 12th internationl biodeterioration and biodegradation symposium (bisosorption and biodremediaton III ). Praha: JMP Tisk s.r.o., 2002, p. 56. Programme, Abstract book. ISBN 80-86313-08-5.

@inproceedings{405060,
   author = {Navrátilová, Jitka and Kotoučková, Ludmila and Němec, Miroslav and Neča, Jiří},
   address = {Praha},
   booktitle = {12th internationl biodeterioration and biodegradation symposium (bisosorption and biodremediaton III )},
   keywords = {Rhodococcus opacus; nitroaromatic compounds; degradation},
   language = {eng},
   location = {Praha},
   isbn = {80-86313-08-5},
   pages = {56-56},
   publisher = {JMP Tisk s.r.o.},
   title = {Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds},
   year = {2002}
}

TY  - JOUR
ID  - 405060
AU  - Navrátilová, Jitka - Kotoučková, Ludmila - Němec, Miroslav - Neča, Jiří
PY  - 2002
TI  - Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds
VL  - Programme, Abstract book
PB  - JMP Tisk s.r.o.
CY  - Praha
SN  - 8086313085
KW  - Rhodococcus opacus
KW  - nitroaromatic compounds
KW  - degradation
N2  - Nitroaromatic compounds are released into the environment trough their wide use as dyes, plasticizers, pesticides, explozives and solvents. The presence of the nitro group causes such compounds to be more resistant to biodegradation than the unsubstituted analogs.The rhodococci have attracted and increased interest in the biochemical and genetical characterization of their metabolic capabilities, based on their ability to transform or degrade diverse classes of mainly hydrophobic substances. Rhodococcus opacus J3 is capable to degrade nitroaromatic compounds.This strain was isolated from soil by selectively enrichment with 4-nitrocatechol in mineral medium. Rhodococcus opacus J3 is gram-positive, strict aerobic bacterium who exhibit a typical rod-coccus cycle.This bacterium was identified as Rhodococcus opacus on the base of morfology, physiology, biochemical characterization, whole cell fatty acid composition and determination of partial sequence of genes for 16S rRNA. Biodegradation of 9 nitroaromatics was tested.We determined degradation of 5 substrates:4-nitrocatechol, 4-nitroguaiacol, 5-nitroguaiacol, 3-nitrophenol and 2,4-dinitrobenzoic acid. We tested degradation in concentration range from 0,025 mM to 0,1 mM. We did not determine degradation of 2-nitrophenol, 4-nitrophenol, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-diphluoro-6- nitrophenol. Concentration of nitroaromatics and nitrites released were measured spectrophotometricaly. Degradation of substrates was confirmed by HPLC. The process of degradation was monitored on automatic microbiological system Bioscreen C.
ER  -

NAVRÁTILOVÁ, Jitka, Ludmila KOTOUČKOVÁ, Miroslav NĚMEC and Jiří NEČA. Rhodococcus opacus J3 - the bacterium degrading nitroaromatic compounds. In \textit{12th internationl biodeterioration and biodegradation symposium (bisosorption and biodremediaton III )}. Praha: JMP Tisk s.r.o., 2002, p.~56. Programme, Abstract book. ISBN~80-86313-08-5.

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