Další formáty:
BibTeX
LaTeX
RIS
@article{405489, author = {Padrta, Petr and Štefl, Richard and Králík, Lukáš and Žídek, Lukáš and Sklenář, Vladimír}, article_location = {Dordrecht}, article_number = {1}, keywords = {DNA hairpin; NMR; refinement; residual dipolar couplings}, language = {eng}, issn = {0925-2738}, journal = {Journal of Biomolecular NMR}, title = {Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings}, volume = {24}, year = {2002} }
TY - JOUR ID - 405489 AU - Padrta, Petr - Štefl, Richard - Králík, Lukáš - Žídek, Lukáš - Sklenář, Vladimír PY - 2002 TI - Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings JF - Journal of Biomolecular NMR VL - 24 IS - 1 SP - 1 EP - 1 PB - Kluwer/Escom SN - 09252738 KW - DNA hairpin KW - NMR KW - refinement KW - residual dipolar couplings N2 - The structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by NMR spectroscopy and refined using molecular dynamics with NOE-derived distances, torsion angles, and residual dipolar couplings (RDCs), is presented. Although the studied molecule is of small size, it is demonstrated that the incorporation of diminutive RDCs can significantly improve local structure determination of regions undefined by the conventional restraints. Very good correlation between the experimental and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C, and 13C-15N coupling constants has been attained. The final structures clearly show typical features of the miniloop architecture. The structure is discussed in context of the extraordinary stability of the d(GCGAAGC) hairpin, which originates from a complex interplay between the aromatic base stacking and hydrogen bonding interactions. ER -
PADRTA, Petr, Richard ŠTEFL, Lukáš KRÁLÍK, Lukáš ŽÍDEK a Vladimír SKLENÁŘ. Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings. \textit{Journal of Biomolecular NMR}. Dordrecht: Kluwer/Escom, 2002, roč.~24, č.~1, s.~1-14. ISSN~0925-2738.
|