| BENEŠ, Petr, G. KOELSCH, B. DVOŘÁK, M. FUSEK a V. VETVICKA. Detection of procathepsin D in rat milk. Comparative Biochemistry and Physiology B - Biochemistry & Molecular Biology. ELSEVIER SCIENCE, 2002, roč. 2002, č. 133, s. 113-118. ISSN 1096-4959. |
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@article{405577,
author = {Beneš, Petr and Koelsch, G. and Dvořák, B. and Fusek, M. and Vetvicka, V.},
article_number = {133},
keywords = {Aspartic protease; Cathepsin D; Procathepsin D; Rat milk; Zymogen},
language = {eng},
issn = {1096-4959},
journal = {Comparative Biochemistry and Physiology B - Biochemistry & Molecular Biology},
title = {Detection of procathepsin D in rat milk},
volume = {2002},
year = {2002}
}
TY - JOUR
ID - 405577
AU - Beneš, Petr - Koelsch, G. - Dvořák, B. - Fusek, M. - Vetvicka, V.
PY - 2002
TI - Detection of procathepsin D in rat milk
JF - Comparative Biochemistry and Physiology B - Biochemistry & Molecular Biology
VL - 2002
IS - 133
SP - 113
EP - 113
PB - ELSEVIER SCIENCE
SN - 10964959
KW - Aspartic protease
KW - Cathepsin D
KW - Procathepsin D
KW - Rat milk
KW - Zymogen
N2 - The presence of procathepsin D, a zymogen of the soluble lysosomal aspartic proteinase cathepsin D, was detected in rat milk using Western blot analysis and assay of proteolytic activity in acidic buffers. No other forms of cathepsin D were found. Two different polyclonal anti-procathepsin D antibodies were used for immunochemical detection of procathepsin D. Both antibodies we found to recognize rat procathepsin D. Proteolytic activity in acidic buffers was detected using a fluorogenic substrate specific for cathepsin D and was abolished by pepstatin A, a specific inhibitor of aspartic proteinases. This study represents third demonstration of presence of procathepsin D in mammal breast milk. Potential sources and physiological functions are discussed.
ER -
BENEŠ, Petr, G. KOELSCH, B. DVOŘÁK, M. FUSEK a V. VETVICKA. Detection of procathepsin D in rat milk. \textit{Comparative Biochemistry and Physiology B - Biochemistry \&{} Molecular Biology}. ELSEVIER SCIENCE, 2002, roč.~2002, č.~133, s.~113-118. ISSN~1096-4959.
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