Ergosterol induces mobilization of internal calcium in tobacco cells

KAŠPAROVSKÝ, Tomáš, ML MILAT, JP BLEIN and Vladimír MIKEŠ. Ergosterol induces mobilization of internal calcium in tobacco cells. In Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci. Prague: Institute of agricultural an food inforamtion, 2002, p. 516-518. ISSN 1212-2580.

Basic information

Original name

Ergosterol induces mobilization of internal calcium in tobacco cells

Authors

KAŠPAROVSKÝ, Tomáš (203 Czech Republic), ML MILAT (250 France), JP BLEIN (250 France) and Vladimír MIKEŠ (203 Czech Republic, guarantor)

Edition

Prague, Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci, p. 516-518, 3 pp. 2002

Publisher

Institute of agricultural an food inforamtion

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/02:00006495

Organization unit

Faculty of Science

ISSN

Keywords in English

ergposterol; plant protection; tobacco; elicitation

Tags

elicitation, ergposterol, plant protection, tobacco

Tags

International impact

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Abstract

V originále

Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases.

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Links

GA522/02/0925, research and development project	Name: Modelování struktury a interakce bílkovinných elicitorů houby Phytophthora Investor: Czech Science Foundation, Modeling of the structure and interaction of elicitors of the fungus Phytophthora
ME 321, research and development project	Name: Studium účinku elicitinů vyvolávajících obrannou reakci u rostlin. Investor: Ministry of Education, Youth and Sports of the CR, Study of the effects of elicitins stimulating a defence reacions in plants.
MSM 143100005, plan (intention)	Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
NF5991, research and development project	Name: Genetika závislostí: zjišťování vztahu polymorfizmů kandidátních genů k alkoholizmu Investor: Ministry of Health of the CR, Genetic study of alcohol dependence : detection of the association between candidate gene polymorphisms and alcoholism.