2002
Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes
FALK, Martin, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK a Michal KOZUBEKZákladní údaje
Originální název
Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes
Autoři
FALK, Martin (203 Česká republika), Emilie LUKÁŠOVÁ (203 Česká republika), Stanislav KOZUBEK (203 Česká republika, domácí) a Michal KOZUBEK (203 Česká republika, garant, domácí)
Vydání
Gene, Amsterdam, Elsevier, 2002, 0378-1119
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.778
Kód RIV
RIV/00216224:14330/02:00006556
Organizační jednotka
Fakulta informatiky
UT WoS
000177085300002
Klíčová slova anglicky
nuclear architecture; chromosome X inactivation; three dimensional fluorescence in situ hybridization; confocal microscopy; chromatin; condensation
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 24. 8. 2012 12:24, doc. RNDr. Martin Falk, Ph.D.
Anotace
V originále
Topography of three genetic elements - dystrophin (dmd) exons 5-7 (E-1), 46-47 (E-2) and centromere of chromosome X (N-x) were studied relative to cell nuclei and to chromosome X territories of spatially fixed human lymphocytes. Repeated three-dimensional (3D) dual color fluorescence in situ hybridization combined with high-resolution cytometry was used. In addition, the nuclear location of fluorescence weight centers (FWC), spatial volume, and maximal area per one section of chromosome-X territories were investigated. The larger (X-L) and smaller (X-s) homologous X-chromosomes were distinguished for each nucleus according to the 3D volume of their territories. The distributions of the [center of nucleus]-to-[genetic element] distances (radial distributions) of dmd exons E-1, E-2, centromere N-x and FWC were very similar for both homologous X-chromosomes of female lymphocytes as well as for the chromosome X of the human male. On the other hand, larger average mutual distances between all pairs of signals (E-1, E-2, N-x, FWC) and larger average maximal area were observed for the larger chromosome (X-L) in comparison with the smaller one (X-s). The territory of the larger homologue showed also more irregular surface. The most significant differences between homologous X-chromosomes were found for N-x-E-1, N-x-E-2 and E-1-E-2 distances that were in average about twice longer for X-L as compared with X-s. These parameters correlate to each other and can be used for the reliable determination of more (de)condensed X-chromosome territory. The longer E-1-E-2 distances for X-L indicate more open chromatin structure of the dystrophin gene on this chromosome in contrary to closed structure on X-s. Substantially shorter distances of the dystrophin exons from the centromeric heterochromatin in X-s as compared to X-L can be explained by silencing effect of centromeres as described in Nature 1 (2000) 137.
Návaznosti
| GA301/01/0186, projekt VaV |
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| IBS5004010, projekt VaV |
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| NC5955, projekt VaV |
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