2002
The 3D structure of human chromosomes in cell nuclei
LUKÁŠOVÁ, Emilie; Stanislav KOZUBEK; Michal KOZUBEK; Martin FALK; Jana AMRICHOVÁ et. al.Základní údaje
Originální název
The 3D structure of human chromosomes in cell nuclei
Autoři
LUKÁŠOVÁ, Emilie (203 Česká republika); Stanislav KOZUBEK (203 Česká republika, domácí); Michal KOZUBEK (203 Česká republika, garant, domácí); Martin FALK (203 Česká republika) a Jana AMRICHOVÁ (203 Česká republika, domácí)
Vydání
Chromosome Research, Dordrecht, Kluwer Academic, 2002, 0967-3849
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.828
Kód RIV
RIV/00216224:14330/02:00006557
Organizační jednotka
Fakulta informatiky
UT WoS
000178954300002
Klíčová slova anglicky
chromosome structure; confocal microscopy; mathematical models; nuclear architecture
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 24. 8. 2012 12:24, doc. RNDr. Martin Falk, Ph.D.
Anotace
V originále
The spatial arrangement of some genetic elements relative to chromosome territories and in parallel with the cell nucleus was investigated in human lymphocytes. The structure of the chromosome territories was studied in chromosomes containing regions (clusters) of highly expressed genes (HSA 9, 17) and those without such clusters (HSA 8, 13). In chromosomes containing highly expressed regions, the elements pertaining to these regions were found close to the centre of the nucleus on the inner sides of chromosome territories; those pertaining to regions with low expression were localized close to the nuclear membrane on the opposite sides of the territories. In chromosomes with generally low expression (HSA 8, 13), the elements investigated were found symmetrically distributed over the territories. Based on the investigations of the chromosome structure, the following conclusions are suggested: (1) Chromosome territories have a non-random internal 3D structure with defined average mutual positions between elements. For example, RARalpha, TP53 and Iso-q of HSA 17 are nearer to each other than they are to the HSA 17 centromere. (2) The structure of a chromosome territory reflects the number and chromosome location of clusters of highly expressed genes. (3) Chromosome territories behave to some extent as solid bodies: if the territory is found closer to the nuclear centre, the individual genetic elements of this chromosome are also found, on average, closer the centre of the nucleus. (4) The positions of centromeres are, on average, nearer to the fluorescence weight centre of the territory (FWCT) than to genes. (5) Active genes are not found near the centromeres of their own territory. A simple model of the structure of chromosome territory is proposed.
Návaznosti
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