Polymorphism in the +252(A/G) lymphotoxin-alpha and the -308(A/G) tumor necrosis factor-alpha genes and susceptibility to chronic periodontitis in a Czech population

FASSMANN, Antonín, Lydie IZAKOVIČOVÁ HOLLÁ, Dana BUČKOVÁ, Anna VAŠKŮ, Vladimír ZNOJIL and Jiří VANĚK. Polymorphism in the +252(A/G) lymphotoxin-alpha and the -308(A/G) tumor necrosis factor-alpha genes and susceptibility to chronic periodontitis in a Czech population. J Periodontal Res. Denmark: Munksgaard, 2003, vol. 38, No 4, p. 394-399. ISSN 0022-3484.

Basic information

• Original name: Polymorphism in the +252(A/G) lymphotoxin-alpha and the -308(A/G) tumor necrosis factor-alpha genes and susceptibility to chronic periodontitis in a Czech population
• Authors: FASSMANN, Antonín (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor), Dana BUČKOVÁ (203 Czech Republic), Anna VAŠKŮ (203 Czech Republic), Vladimír ZNOJIL (203 Czech Republic) and Jiří VANĚK (203 Czech Republic).
• Edition: J Periodontal Res, Denmark, Munksgaard, 2003, 0022-3484.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Denmark
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.407
• RIV identification code: RIV/00216224:14110/03:00008562
• Organization unit: Faculty of Medicine
• UT WoS: 000184143100007
• Keywords in English: periodontitis; gene; polymorphism; lymphotoxin; TNF
• Tags: gene, lymphotoxin, periodontitis, polymorphism, TNF

Abstract

Periodontitis is an inflammatory disease that leads to irreversible attachment loss, bone destruction and eventually tooth loss. Tumor necrosis factor (TNF), a pluripotent proinflammatory cytokine that is able to induce tissue destruction and bone resorption, has been implicated in the pathogenesis of periodontal disease. In this study, we investigated an association between chronic periodontitis and two previously described bi-allelic polymorphisms in the TNF locus: a G to A transition at position -308 in the 5'promoter region of the TNF-alpha gene and an NcoI restriction fragment length polymorphism (RFLP) in the first intron (position +252 A/G) of the lymphotoxin alpha (LT-alpha) gene. The TNF-alpha (-308G/A) polymorphism itself showed no association with chronic periodontitis, whereas the frequency distribution of the LT-alpha (+252A/G) genotypes showed statistically significant differences between patients and the reference group. The proportion of individuals carrying the LT-alpha 1/1 genotype was significantly lower in the group of patients with chronic periodontitis (0.8%) than in the control group (8.8%), (P < 0.0094, Pcorr < 0.05). However, the significant differences in the frequencies of the combined genotypes (TNF-alpha and LT-alpha) between the control and the patient groups were found using a simulation by applying the Monte-Carlo method (p<0.01). Conclusion: Our data suggest that combined genotypes composed of the TNF-alpha and LT-alpha gene polymorphisms may influence the susceptibility to chronic periodontitis. We also showed that, comparing the two genes, the 1/1 genotype of the NcoI polymorphism in the first intron of the LT-alpha gene is a more informative marker and it may be one of the protective genetic factors against chronic periodontitis in our population.

Links

• MSM 141100002, plan (intention): Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases