Occurrence of antibiotic-resistant bacterial strains isolated
in poultry

J 2002

Occurrence of antibiotic-resistant bacterial strains isolated in poultry

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ et. al.

Basic information

Original name

Occurrence of antibiotic-resistant bacterial strains isolated in poultry

Authors

KOLÁŘ, Milan (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, guarantor), Jan BARDOŇ (203 Czech Republic), Iva VÁGNEROVÁ (203 Czech Republic), H. TYPOVSKÁ (203 Czech Republic), Ivo VÁLKA (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Veterinary Medicine - Czech, Brno, Veterinary Research Institute Brno, 2002, 0375-8427

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

[Full Text]

Impact factor

Impact factor: 0.204

RIV identification code

RIV/00216224:14310/02:00006631

Organization unit

Faculty of Science

UT WoS

000175865800004

Keywords in English

Vancomycin Resistance; Enterococcus; Staphylococcus; Pulsed Field-Gel Electrophoreris; Plasmid Analysis; Poultry

Abstract

V originále

The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent both gramnegatives and grampositives, both the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance (in 10% of the strains) were found also against ofloxacin and ciprofloxacin. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant against vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged among Enterococcus sp. group III VanB. Remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, vancomycin-resistant Enterococcus faecium strains, regardless of their resistance type, formed a cluster distinct from control group of vancomycin sensitive strains. Furthermore, among the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogenous.

Links

GA301/99/D075, research and development project

Name: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Czech Science Foundation, Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ, Ivo VÁLKA and Jiří DOŠKAŘ. Occurrence of antibiotic-resistant bacterial strains isolated in poultry. Veterinary Medicine - Czech. Brno: Veterinary Research Institute Brno, 2002, vol. 47, 2-3, p. 52-59. ISSN 0375-8427.

@article{406330,
   author = {Kolář, Milan and Pantůček, Roman and Bardoň, Jan and Vágnerová, Iva and Typovská, H. and Válka, Ivo and Doškař, Jiří},
   article_location = {Brno},
   article_number = {2-3},
   keywords = {Vancomycin Resistance; Enterococcus; Staphylococcus; Pulsed Field-Gel Electrophoreris; Plasmid Analysis; Poultry},
   language = {eng},
   issn = {0375-8427},
   journal = {Veterinary Medicine - Czech},
   title = {Occurrence of antibiotic-resistant bacterial strains isolated in poultry},
   url = {http://www.vri.cz/docs/vetmed/47-3-52.pdf},
   volume = {47},
   year = {2002}
}

TY  - JOUR
ID  - 406330
AU  - Kolář, Milan - Pantůček, Roman - Bardoň, Jan - Vágnerová, Iva - Typovská, H. - Válka, Ivo - Doškař, Jiří
PY  - 2002
TI  - Occurrence of antibiotic-resistant bacterial strains isolated in poultry
JF  - Veterinary Medicine - Czech
VL  - 47
IS  - 2-3
SP  - 52
EP  - 52
PB  - Veterinary Research Institute Brno
SN  - 03758427
KW  - Vancomycin Resistance
KW  - Enterococcus
KW  - Staphylococcus
KW  - Pulsed Field-Gel Electrophoreris
KW  - Plasmid Analysis
KW  - Poultry
UR  - http://www.vri.cz/docs/vetmed/47-3-52.pdf
N2  - The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent both gramnegatives and grampositives, both the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance (in 10% of the strains) were found also against ofloxacin and ciprofloxacin. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant against vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged among Enterococcus sp. group III VanB. Remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, vancomycin-resistant Enterococcus faecium strains, regardless of their resistance type, formed a cluster distinct from control group of vancomycin sensitive strains. Furthermore, among the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogenous.
ER  -

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ, Ivo VÁLKA and Jiří DOŠKAŘ. Occurrence of antibiotic-resistant bacterial strains isolated in poultry. \textit{Veterinary Medicine - Czech}. Brno: Veterinary Research Institute Brno, 2002, vol.~47, 2-3, p.~52-59. ISSN~0375-8427.

Detailed Information on Publication Record