Genomic and phenotypic variability of multiresistant strains
Staphylococcus hominis subsp. novobiosepticus

D 2002

Genomic and phenotypic variability of multiresistant strains Staphylococcus hominis subsp. novobiosepticus

PETRÁŠ, Petr, Roman PANTŮČEK a Jiří DOŠKAŘ

Základní údaje

Originální název

Genomic and phenotypic variability of multiresistant strains Staphylococcus hominis subsp. novobiosepticus

Autoři

PETRÁŠ, Petr (203 Česká republika), Roman PANTŮČEK (203 Česká republika, garant) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Tsukuba, Japan, 10th International Symposium on Staphylococci and Staphylococcal Infections, s. 65-65, 2002

Nakladatel

Juntendo University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Japonsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006632

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Antibiotic Resistance; Staphylococcus hominis subsp. novobiosepticus; Bacterial Typing Techniques; DNA; Pulsed Field-Gel Electrophoresis

Štítky

Antibiotic Resistance, Bacterial Typing Techniques, DNA, Pulsed Field-Gel Electrophoresis, Staphylococcus hominis subsp. novobiosepticus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Background.In 1998,a new subspecies,Staphylococcus hominis subsp.novobiosepticus (SHN),isolated from human clinical material,was described by Kloos,et al.We present genetic and phenotypic characteristics of 192 SHN strains,isolated in 24 cities of the Czech Republic (CR). Methods.A total of 192 SHN strains were collected in 29 Czech hospitals mainly from the blood (72%).They were tested for resistance to 12 antibiotics by the disk-diffusion method.Molecular typing was carried out to map characteristics of 70 selected SHN strains.To study possible genetic relatedness between strains,the Sma I- and Ksp I-macrorestriction analysis,plasmid content analysis and AP-PCR were used. Results.Multiresistant and virulent strains showed practically identical phenotypic characteristics as type strain CCM 4786 T .The most refined genotyping results were obtained by macrorestriction analysis,differentiating unambiguously between SHN and other S.hominis subspecies at a macrorestricton pattern similarity level of 25%.In SHN strains,32 different macrorestriction patterns with similarity range of 35%-95%were identified. Based on the cluster analysis,the strains were divided into 14 clonal types. Conclusions.Particular attention should be focused on the incidence of SHN strains,thought to play an important part in hospital infections,mainly those of the bloodstream.

Návaznosti

GA301/02/1505, projekt VaV

Název: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Grantová agentura ČR, Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

PETRÁŠ, Petr, Roman PANTŮČEK a Jiří DOŠKAŘ. Genomic and phenotypic variability of multiresistant strains Staphylococcus hominis subsp. novobiosepticus. In 10th International Symposium on Staphylococci and Staphylococcal Infections. Tsukuba, Japan: Juntendo University, 2002, s. 65.

@inproceedings{406332,
   author = {Petráš, Petr and Pantůček, Roman and Doškař, Jiří},
   address = {Tsukuba, Japan},
   booktitle = {10th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Antibiotic Resistance; Staphylococcus hominis subsp. novobiosepticus; Bacterial Typing Techniques; DNA; Pulsed Field-Gel Electrophoresis},
   language = {eng},
   location = {Tsukuba, Japan},
   pages = {65-65},
   publisher = {Juntendo University},
   title = {Genomic and phenotypic variability of multiresistant strains Staphylococcus hominis subsp. novobiosepticus},
   year = {2002}
}

TY  - JOUR
ID  - 406332
AU  - Petráš, Petr - Pantůček, Roman - Doškař, Jiří
PY  - 2002
TI  - Genomic and phenotypic variability of multiresistant strains Staphylococcus hominis subsp. novobiosepticus
PB  - Juntendo University
CY  - Tsukuba, Japan
KW  - Antibiotic Resistance
KW  - Staphylococcus hominis subsp. novobiosepticus
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Pulsed Field-Gel Electrophoresis
N2  - Background.In 1998,a new subspecies,Staphylococcus hominis subsp.novobiosepticus (SHN),isolated from human clinical material,was described by Kloos,et al.We present genetic and phenotypic characteristics of 192 SHN strains,isolated in 24 cities of the Czech Republic (CR). Methods.A total of 192 SHN strains were collected in 29 Czech hospitals mainly from the blood (72%).They were tested for resistance to 12 antibiotics by the disk-diffusion method.Molecular typing was carried out to map characteristics of 70 selected SHN strains.To study possible genetic relatedness between strains,the Sma I- and Ksp I-macrorestriction analysis,plasmid content analysis and AP-PCR were used. Results.Multiresistant and virulent strains showed practically identical phenotypic characteristics as type strain CCM 4786 T .The most refined genotyping results were obtained by macrorestriction analysis,differentiating unambiguously between SHN and other S.hominis subspecies at a macrorestricton pattern similarity level of 25%.In SHN strains,32 different macrorestriction patterns with similarity range of 35%-95%were identified. Based on the cluster analysis,the strains were divided into 14 clonal types. Conclusions.Particular attention should be focused on the incidence of SHN strains,thought to play an important part in hospital infections,mainly those of the bloodstream.
ER  -

PETRÁŠ, Petr, Roman PANTŮČEK a Jiří DOŠKAŘ. Genomic and phenotypic variability of multiresistant strains $<$I$>$Staphylococcus hominis$<$/I$>$ subsp. $<$I$>$novobiosepticus$<$/I$>$. In \textit{10th International Symposium on Staphylococci and Staphylococcal Infections}. Tsukuba, Japan: Juntendo University, 2002, s.~65.

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