| RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Jiří LENFELD, Ivo RUDOLF, Daniel HORÁK and Milan BENEŠ. Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles. Journal of Chromatography B. Amsterdam: Elsevier, 2002, vol. 2002, No 774, p. 25-31. ISSN 1570-0232. |
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@article{406440,
author = {Rittich, Bohuslav and Španová, Alena and Ohlashennyy, Yuriy and Lenfeld, Jiří and Rudolf, Ivo and Horák, Daniel and Beneš, Milan},
article_location = {Amsterdam},
article_number = {774},
keywords = {Magnetic bead cellulose particles; magnetic poly(HEMA-co-EDMA); deoxyribonuclease I},
language = {eng},
issn = {1570-0232},
journal = {Journal of Chromatography B},
title = {Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles},
volume = {2002},
year = {2002}
}
TY - JOUR
ID - 406440
AU - Rittich, Bohuslav - Španová, Alena - Ohlashennyy, Yuriy - Lenfeld, Jiří - Rudolf, Ivo - Horák, Daniel - Beneš, Milan
PY - 2002
TI - Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles
JF - Journal of Chromatography B
VL - 2002
IS - 774
SP - 25-31
EP - 25-31
PB - Elsevier
SN - 15700232
KW - Magnetic bead cellulose particles
KW - magnetic poly(HEMA-co-EDMA)
KW - deoxyribonuclease I
N2 - Magnetic bead cellulose particles and magnetic poly(HEMA-co-EDMA) microsheres with immobilized DNaseI were used for degradation of chromosomal and plasmid DNAs. Divalent cations (Mg2+, Ca2+, Mn2+ and Co2+) were used for the activation of DNaseI. A comparison of free and immobilized enzyme (magnetic bead particles) activities was carried out in dependence on pH and activating cation. The maximum of the activity of immobilized DNaseI was shifted to lower pH compared with free DNase I. DNase I immobilized on magnetic bead cellulose was used 20 times in the degradation of chromosomal DNA. Its residual activity was influenced by the nature of activating divalent cation. The immobilized enzyme with decreased activity was reactivated by Co2+ ions.
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Jiří LENFELD, Ivo RUDOLF, Daniel HORÁK and Milan BENEŠ. Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles. \textit{Journal of Chromatography B}. Amsterdam: Elsevier, 2002, vol.~2002, No~774, p.~25-31. ISSN~1570-0232.
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