Spectrin-like protein in yeasts

D 2002

Spectrin-like protein in yeasts

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA

Základní údaje

Originální název

Spectrin-like protein in yeasts

Autoři

SLANINOVÁ, Iva (203 Česká republika, garant), Alena HOLUBÁŘOVÁ (203 Česká republika) a Augustin SVOBODA (203 Česká republika)

Vydání

Bratislava, Sborník XXX th Annual Conference on yeasts, s. 36-36, 2002

Nakladatel

Československá mikrobiologická společnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/02:00006734

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

actin; spectrin; yeast; cytoskeleton

Štítky

Actin, Cytoskeleton, spectrin, Yeast

Změněno: 6. 5. 2003 16:27, prof. MUDr. Iva Slaninová, Ph.D.

Anotace

V originále

Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface.

Návaznosti

GA204/00/0394, projekt VaV

Název: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Grantová agentura ČR, Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

GA204/99/D025, projekt VaV

Název: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Grantová agentura ČR, Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Citovat

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA. Spectrin-like protein in yeasts. In Sborník XXX th Annual Conference on yeasts. Bratislava: Československá mikrobiologická společnost, 2002, s. 36.

@inproceedings{406736,
   author = {Slaninová, Iva and Holubářová, Alena and Svoboda, Augustin},
   address = {Bratislava},
   booktitle = {Sborník XXX th Annual Conference on yeasts},
   keywords = {actin; spectrin; yeast; cytoskeleton},
   language = {eng},
   location = {Bratislava},
   pages = {36-36},
   publisher = {Československá mikrobiologická společnost},
   title = {Spectrin-like protein in yeasts},
   year = {2002}
}

TY  - JOUR
ID  - 406736
AU  - Slaninová, Iva - Holubářová, Alena - Svoboda, Augustin
PY  - 2002
TI  - Spectrin-like protein in yeasts
PB  - Československá mikrobiologická společnost
CY  - Bratislava
KW  - actin
KW  - spectrin
KW  - yeast
KW  - cytoskeleton
N2  - Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface.
ER  -

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA. Spectrin-like protein in yeasts. In \textit{Sborník XXX th Annual Conference on yeasts}. Bratislava: Československá mikrobiologická společnost, 2002, s.~36.

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