Optimisation of Immunohistochemical Detection of Cytokinins in
Transgenic Arabidopsis Seedlings Expressing Activated ipt, a
Cytokinin Biosynthesis Gene

D 2002

Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene

DUBOVÁ, Jaroslava, Julie Olegivna ROTKOVSKÁ, Alena REKOVÁ a Břetislav BRZOBOHATÝ

Základní údaje

Originální název

Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene

Název česky

Optimalizace imunohistochemické detekce cytokininů u transgenních klíčních rostlin Arabidopsis exprimujících ipt, gen pro biosyntézu cytokininů

Autoři

DUBOVÁ, Jaroslava (203 Česká republika), Julie Olegivna ROTKOVSKÁ (804 Ukrajina), Alena REKOVÁ (203 Česká republika) a Břetislav BRZOBOHATÝ (203 Česká republika, garant)

Vydání

Brno, Progress in Basic, Applied and Diagnostic Histochemistry, s. 15-15, 2002

Nakladatel

Masarykova univerzita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006761

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-2998-6

Klíčová slova anglicky

cytokinins; immunohistochemistry; Arabidopsis

Štítky

Arabidopsis, cytokinins, Immunohistochemistry

Změněno: 7. 3. 2006 18:57, RNDr. Jaroslava Dubová, CSc.

Anotace

V originále

Aseptically grown Arabidopsis seedlings were collected, fixed in PFA - GA mixture. Indirect immunohistochemistry on cryostat sections was used. Primary antibodies against zeatin-riboside and isopentenyladenine-riboside were used respectively. Goat anti-rabbit IgG conjugated with AP was used as a secondary antibody. AP activity was detected by reaction with BCIP and NBT. The best results of immunostaining were obtained by higher dilution of primary antibodies and lowering the concentration of BCIP and NBT. Arabidopsis seedlings with activated ipt showed higher the amount of reaction product in comparison with the wild type plants. Accumulation of cytokinins was established immunohistochemically during culture period in transgenic Arabidopsis seedlings, which is in accordance with the biochemical analyses. Modified immunohistochemical detection of free bases of cytokinins can be used for the semi quantitative evaluation of cytokinin content in Arabidopsis seedlings.

Návaznosti

IAA5004001, projekt VaV

Název: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Investor: Akademie věd ČR, Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Citovat

DUBOVÁ, Jaroslava, Julie Olegivna ROTKOVSKÁ, Alena REKOVÁ a Břetislav BRZOBOHATÝ. Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene. In Progress in Basic, Applied and Diagnostic Histochemistry. Brno: Masarykova univerzita v Brně, 2002, s. 15. ISBN 80-210-2998-6.

@inproceedings{406823,
   author = {Dubová, Jaroslava and Rotkovská, Julie Olegivna and Reková, Alena and Brzobohatý, Břetislav},
   address = {Brno},
   booktitle = {Progress in Basic, Applied and Diagnostic Histochemistry},
   keywords = {cytokinins; immunohistochemistry; Arabidopsis},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2998-6},
   pages = {15-15},
   publisher = {Masarykova univerzita v Brně},
   title = {Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene},
   year = {2002}
}

TY  - JOUR
ID  - 406823
AU  - Dubová, Jaroslava - Rotkovská, Julie Olegivna - Reková, Alena - Brzobohatý, Břetislav
PY  - 2002
TI  - Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene
PB  - Masarykova univerzita v Brně
CY  - Brno
SN  - 8021029986
KW  - cytokinins
KW  - immunohistochemistry
KW  - Arabidopsis
N2  - Aseptically grown Arabidopsis seedlings were collected, fixed in PFA - GA mixture. Indirect immunohistochemistry on cryostat sections was used. Primary antibodies against zeatin-riboside and isopentenyladenine-riboside were used respectively. Goat anti-rabbit IgG conjugated with AP was used as a secondary antibody. AP activity was detected by reaction with BCIP and NBT. The best results of immunostaining were obtained by higher dilution of primary antibodies and lowering the concentration of BCIP and NBT. Arabidopsis seedlings with activated ipt showed higher the amount of reaction product in comparison with the wild type plants. Accumulation of cytokinins was established immunohistochemically during culture period in transgenic Arabidopsis seedlings, which is in accordance with the biochemical analyses. Modified immunohistochemical detection of free bases of cytokinins can be used for the semi quantitative evaluation of cytokinin content in Arabidopsis seedlings.
ER  -

DUBOVÁ, Jaroslava, Julie Olegivna ROTKOVSKÁ, Alena REKOVÁ a Břetislav BRZOBOHATÝ. Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene. In \textit{Progress in Basic, Applied and Diagnostic Histochemistry}. Brno: Masarykova univerzita v Brně, 2002, s.~15. ISBN~80-210-2998-6.

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