Quantitative immunofluorescence for BDNF, NGF, and NT-3 in
Schwann cells of the spinal roots and peripheral nerve

D 2002

Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ

Základní údaje

Originální název

Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve

Autoři

DUBOVÝ, Petr (203 Česká republika, garant), Ilona KLUSÁKOVÁ (203 Česká republika) a Ivana SVÍŽENSKÁ (203 Česká republika)

Vydání

Brno, Progress in Basic, Applied, and Diagnostic Histochemistry, s. 16-16, 2002

Nakladatel

Masarykova univerzita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/02:00006844

Organizační jednotka

Lékařská fakulta

ISBN

80-210-2998-6

Klíčová slova anglicky

neurotrophins; immunofluorescence; image analysis

Štítky

Image analysis, immunofluorescence, neurotrophins

Příznaky

Mezinárodní význam

Změněno: 17. 6. 2009 10:55, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence intensity for BDNF, NGF and NT-3 was higher in the Schwann cells of cutaneous than motor branches of the intact femoral nerve. On the other hand, a higher intensity for BDNF and NT-3 immunofluorescence was measured in the Schwann cells of the intact dorsal than ventral roots while no difference was found for NFG immunostaining. Two weeks after rhizotomy or nerve transection immunofluorescence intensity for investigated neurotrophins was significantly higher in all distal Schwann cells in comparison to the ones of the intact roots or nerves. In addition, intensity of immunofluorescence for BDNF, NGF and NT-3 was very similar in the Schwann cells of both motor and cutaneous branches two weeks after transection of the femoral nerve. Comparing the Schwann cells of rhizotomized roots the immunofluorescence was significantly higher in the ventral than dorsal roots. The results of quantitative immunofluorescence staining revealed that the axotomy-induced up-regulation of neurotrophins is different in the Schwann cells of spinal roots and peripheral nerves. In addition, there is a different up-regulation of neurotrophins in the reactive Schwann cells alongside afferent and motor axons of the spinal roots preparing different condition for their regeneration.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve. In Progress in Basic, Applied, and Diagnostic Histochemistry. Brno: Masarykova univerzita v Brně, 2002, s. 16. ISBN 80-210-2998-6.

@inproceedings{407047,
   author = {Dubový, Petr and Klusáková, Ilona and Svíženská, Ivana},
   address = {Brno},
   booktitle = {Progress in Basic, Applied, and Diagnostic Histochemistry},
   keywords = {neurotrophins; immunofluorescence; image analysis},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2998-6},
   pages = {16-16},
   publisher = {Masarykova univerzita v Brně},
   title = {Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve},
   year = {2002}
}

TY  - JOUR
ID  - 407047
AU  - Dubový, Petr - Klusáková, Ilona - Svíženská, Ivana
PY  - 2002
TI  - Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve
PB  - Masarykova univerzita v Brně
CY  - Brno
SN  - 8021029986
KW  - neurotrophins
KW  - immunofluorescence
KW  - image analysis
N2  - Axotomy induces development of a microenvironment supporting axonal regeneration in the peripheral nervous system. The promotion of axonal regeneration is related with up-regulation of many molecules including neurotrophins in reactive Schwann cells. The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the sensory and motor neurons for axon regeneration, we hypothesize different cellular and extracellular conditions alongside afferent and motor axons. We have optimized indirect immunofluorescence method for quantitative investigation of neurotrophins (BDNF, NGF and NT-3). The cryostat sections (8 ľm) through the intact and 14-day-rhizotomized dorsal and ventral roots as well as intact and 14-day-transected femoral nerves were cut and incubated simultaneously. An intensity of immunofluorescence staining expressed was compared using the computer-assisted image analysis (Lucia-G v 4.21). Immunofluorescence intensity for BDNF, NGF and NT-3 was higher in the Schwann cells of cutaneous than motor branches of the intact femoral nerve. On the other hand, a higher intensity for BDNF and NT-3 immunofluorescence was measured in the Schwann cells of the intact dorsal than ventral roots while no difference was found for NFG immunostaining. Two weeks after rhizotomy or nerve transection immunofluorescence intensity for investigated neurotrophins was significantly higher in all distal Schwann cells in comparison to the ones of the intact roots or nerves. In addition, intensity of immunofluorescence for BDNF, NGF and NT-3 was very similar in the Schwann cells of both motor and cutaneous branches two weeks after transection of the femoral nerve. Comparing the Schwann cells of rhizotomized roots the immunofluorescence was significantly higher in the ventral than dorsal roots. The results of quantitative immunofluorescence staining revealed that the axotomy-induced up-regulation of neurotrophins is different in the Schwann cells of spinal roots and peripheral nerves. In addition, there is a different up-regulation of neurotrophins in the reactive Schwann cells alongside afferent and motor axons of the spinal roots preparing different condition for their regeneration.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. Quantitative immunofluorescence for BDNF, NGF, and NT-3 in Schwann cells of the spinal roots and peripheral nerve. In \textit{Progress in Basic, Applied, and Diagnostic Histochemistry}. Brno: Masarykova univerzita v Brně, 2002, s.~16. ISBN~80-210-2998-6.

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