Evaluation of antioxidative activity of flavonoids and exctract of Scutellaria baicalensis with coulochemical detection

BOCHOŘÁKOVÁ, Hana, Hana PAULOVÁ, Jiří SLANINA and Eva TÁBORSKÁ. Evaluation of antioxidative activity of flavonoids and exctract of Scutellaria baicalensis with coulochemical detection. In Zborník XVIII.Biochemický zjazd. Bratislava: VEDA, vydavatelstvo SAV, 2002, p. 251.

Basic information

• Original name: Evaluation of antioxidative activity of flavonoids and exctract of Scutellaria baicalensis with coulochemical detection
• Authors: BOCHOŘÁKOVÁ, Hana (203 Czech Republic, guarantor), Hana PAULOVÁ (203 Czech Republic), Jiří SLANINA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic).
• Edition: Bratislava, Zborník XVIII.Biochemický zjazd, p. 251-251, 2002.
• Publisher: VEDA, vydavatelstvo SAV

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/02:00006853
• Organization unit: Faculty of Medicine
• Keywords in English: Scutellaria baicalensis; flavonoids; HPLC-EC; antioxidants
• Tags: antioxidants, flavonoids, HPLC-EC, Scutellaria baicalensis

Abstract

In the present study, measuring of hydrodynamic voltammograms using HPLC-ECD was used for the estimation of oxidative potentials of five flavonoids of SCB (B,BG, W, WG, O), and two standards (rutin R, quercetin Q). The compounds with lower potentials (below 400 mV) are regarded as strong antioxidants. Among the individual flavonoids of SCB only B a BG displayed significantly lower values of the first oxidative potentials (50, 150 mV resp.). The HPLC-ECD method was then applied on the analysis of crude extracts from SCB. The peak of BG was absolutely dominant in the chromatogram of the extract, indicating that due to high content and low oxidative potential BG is predominantly responsible for the antioxidative effect of the extract. In conclusion, the HPLC-EC presented here is very suitable for screening of antioxidative components in complex mixtures, especially in plant extracts.

Abstract (in Czech)

V prezentované práci byly měřeny hydrodynamické voltamogramy stanovené pomocí HPLC-ECD a jejich hodnocením byly získány oxidační potenciály pěti flavonoidů z SCB (B, BG, W, WG, O) a dvou standardů flavonoidů (rutinu a quercetinu). Sloučeniny s nižším potenciálem (pod 400mV) jsou považovány za silné antioxidanty. Z flavonoidů izolovaných z SCB pouze B a BG vykazovaly nižší potenciál. Stejným postupem byl proměřen i surový extrakt SCB. Bylo prokázáno, že BG (dominantní pík v chromatogramu extraktu) je převážně odpovědný za antioxidační aktivitu extraktu SCB. Metoda HPLC-ECD je velmi vhodná pro screening antioxidačních látek v komplexních směsích, zvláště v rostlinných extraktech.

Links

• GA303/00/D062, research and development project: Name: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Czech Science Foundation, Bioavailability and mechanism of antioxidant activity of phenolic acids and lignans