THE EFFECTS OF PH, TEMPERATURE, ELECTRON DONOR AND BIOMASS CONCENTRATION ON THE TIME COURSE OF NITRIC OXIDE CONCENTRATION IN THE DENITRIFICATION PATHWAY OF PARACOCCUS DENITRIFICANS

KUNAK, Michal a Igor KUCERA. THE EFFECTS OF PH, TEMPERATURE, ELECTRON DONOR AND BIOMASS CONCENTRATION ON THE TIME COURSE OF NITRIC OXIDE CONCENTRATION IN THE DENITRIFICATION PATHWAY OF PARACOCCUS DENITRIFICANS. In sborník prispevku. 2002. vyd. Brno: Masarykova Univerzita v Brne, 2002, s. 63-145. VI.Pracovní setkání biochemiku; a molekulárních. ISBN 80-210-2777-0.

Základní údaje

• Originální název: THE EFFECTS OF PH, TEMPERATURE, ELECTRON DONOR AND BIOMASS CONCENTRATION ON THE TIME COURSE OF NITRIC OXIDE CONCENTRATION IN THE DENITRIFICATION PATHWAY OF PARACOCCUS DENITRIFICANS
• Název anglicky: THE EFFECTS OF PH, TEMPERATURE, ELECTRON DONOR AND BIOMASS CONCENTRATION ON THE TIME COURSE OF NITRIC OXIDE CONCENTRATION IN THE DENITRIFICATION PATHWAY OF PARACOCCUS DENITRIFICANS
• Autoři: KUNAK, Michal a Igor KUCERA.
• Vydání: 2002. vyd. Brno, sborník prispevku, s. 63-145, VI.Pracovní setkání biochemiku; a molekulárních, 2002.
• Nakladatel: Masarykova Univerzita v Brne

Další údaje

• Typ výsledku: Stať ve sborníku
• Utajení: není předmětem státního či obchodního tajemství
• Organizační jednotka: Přírodovědecká fakulta
• ISBN: 80-210-2777-0
• Klíčová slova anglicky: Paracoccus denitrification oscillation
• Štítky: Paracoccus denitrification oscillation

Anotace

Nitric oxide is produced by the nitrite reducting nitrite reductase and further consumed by nitric oxide reductase. Time course of nitric oxide concentration was monitored amperometrically by a Clark type electrode and simultaneously by using direct inlet mass spectrometry. pH considerably affected the accumulation of NO. Using ascorbate and N,N,N,N, tetramethyl p phenylendiamine (TMPD) as a source of electrons, oscillations of nitric oxide concentration were observed in the pH range of 6 6,4. Nitric oxide did not accumulate at higher pH values, while the accumulation proceeded at lower pH. This coresponds well with pH dependences of the activities of both involved enzymes (pH optimum of nitritreductase = 6.2 and pH optimum of NO reductase = 7.5). Oscillations of nitric oxide concentration can be elicited by a partial inhibition of nitric oxide reductase with the uncoupler carbonyl cyanide m chlorophenylhydrazone (CCCP). Parameters of NO oscillations were found to be influenced by some factors: concentrations of electron donor TMPD or biomass and reaction temperature.

Anotace anglicky

Nitric oxide is produced by the nitrite reducting nitrite reductase and further consumed by nitric oxide reductase. Time course of nitric oxide concentration was monitored amperometrically by a Clark type electrode and simultaneously by using direct inlet mass spectrometry. pH considerably affected the accumulation of NO. Using ascorbate and N,N,N,N, tetramethyl p phenylendiamine (TMPD) as a source of electrons, oscillations of nitric oxide concentration were observed in the pH range of 6 6,4. Nitric oxide did not accumulate at higher pH values, while the accumulation proceeded at lower pH. This coresponds well with pH dependences of the activities of both involved enzymes (pH optimum of nitritreductase = 6.2 and pH optimum of NO-reductase = 7.5). Oscillations of nitric oxide concentration can be elicited by a partial inhibition of nitric oxide reductase with the uncoupler carbonyl cyanide m chlorophenylhydrazone (CCCP). Parameters of NO oscillations were found to be influenced by some factors: concentrations of electron donor TMPD or biomass and reaction temperature.