2002
Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity
FOJTOVÁ, Miloslava, Jana FULNEČKOVÁ, Jiří FAJKUS a Aleš KOVAŘÍKZákladní údaje
Originální název
Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity
Autoři
FOJTOVÁ, Miloslava (203 Česká republika), Jana FULNEČKOVÁ (203 Česká republika), Jiří FAJKUS (203 Česká republika, garant) a Aleš KOVAŘÍK (203 Česká republika)
Vydání
Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2002, 0022-0957
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.852
Kód RIV
RIV/00216224:14310/02:00006960
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000178957500003
Klíčová slova anglicky
apoptosis; cadmium; DNA domain fragmentation; telomerase; tobacco BY-2 cells
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 30. 6. 2009 12:49, prof. RNDr. Jiří Fajkus, CSc.
Anotace
V originále
It has been shown previously that apoptosis of tobacco cells induced by cadmium ions shows a relatively long lag period between exposure and ceel death. This lag phase lasts for 3 d in TBY-2 cell cultures and is characterized by the maintenance of full cell viability despite extensive fragmentation of DNA into pieces of chromatin loop size. Experiments reported here demonstrate that cell death can be prevented if CdSO4 is removed from the growth medium during the lag phase, suggesting that an irreversible apoptotic trigger is delivered within 24 h, between the third and fourth days of cadmium treatment. The post-cadmium recovery phase was characterized by DNA repair at the level of 50-200 kb and increased telomerase activity. Analysis of high-molecular-weight DNA by pulsed-field-gel electrophoresis revealed that the majority of DNA strand breaks was repaired within 48 h after cadmium withdrawal. Telomerase activity increased 2.5-fold in the recovery phase, but elevated levels were also found in cell extracts from apoptotic cells suggesting that telomerase might be associated with DNA repair, but it is not capable of inhibiting ongoing apoptosis. Limited exposure of TBY-2 cells to cadmium elicits non-random DNA damage of relatively high magnitude that can be repaired. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient genotoxic stress.
Návaznosti
MSM 143100008, záměr |
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