KAŠPÁREK, Petr, Jiří DOŠKAŘ and Roman PANTŮČEK. Genome analysis of polyvalent staphylococcal bacteriophage 812. Scripta medica (Brno). Brno: Masaryk University, Faculty of Medicine, 2002, vol. 75, No 1, p. 39. ISSN 1211-3595.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Genome analysis of polyvalent staphylococcal bacteriophage 812
Authors KAŠPÁREK, Petr (203 Czech Republic), Jiří DOŠKAŘ (203 Czech Republic, guarantor) and Roman PANTŮČEK (203 Czech Republic).
Edition Scripta medica (Brno), Brno, Masaryk University, Faculty of Medicine, 2002, 1211-3595.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/02:00007080
Organization unit Faculty of Science
Keywords in English Bacteriophage; Staphylococcus; DNA; Bacteriophage Therapy; Endolysin Gene; Genome Mapping
Tags Bacteriophage, Bacteriophage Therapy, DNA, Endolysin Gene, Genome Mapping, Staphylococcus
Changed by Changed by: prof. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 20/12/2002 13:01.
Abstract
The phage 812 is a polyvalent virulent bacteriophage belonging to the family Myoviridae. It shows strong lytic activity on a broad host range of Staphylococcus aureus subsp.aureus strains as well as on some other staphylococcal species.The phage 812 is closely related to staphylococcal phages U16,SK311 and SK131 classified with the phage species Twort. The object of our study is the analysis of genome regions exhibiting chromosome rearrangements (insertions,deletions and inversions)in the host-range mutants of the phage 812.This analysis should contribute to the explanation of molecular mechanisms leading to the host range changes.Part of this study is focused on identification and characterization of genes and sequences of the phage 812 presumably relating to the host range changes. We determined the nucleotide sequence of a 913 bp insertion identified in the restriction fragment PstI-O of the host-range mutant 812b. The putative gene coding for the major capsid protein of the phage 812 has been found.This protein shows 65 %identity with the major capsid protein of the phage A511 growing on Listeria monocytogenes strains. Hybridization probe prepared from the intron sequences of phage Twort does not hybridize with the phage 812 DNA,nor with that of its host-range mutants,which indicates absence of the introns in these phages.
Links
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
PrintDisplayed: 26/4/2024 11:43