MARŠÁLEK, Petr, Marta FARKOVÁ and Josef HAVEL. Quantitative MALDI TOFMS Analysis of Amino Acids Applying Soft Modeling Methods. Chemical Papers. Bratislava: Slovak Academic Press, 2002, vol. 56, No 3, p. 188-193. ISSN 0366-6352.
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Basic information
Original name Quantitative MALDI TOFMS Analysis of Amino Acids Applying Soft Modeling Methods
Authors MARŠÁLEK, Petr, Marta FARKOVÁ and Josef HAVEL.
Edition Chemical Papers, Bratislava, Slovak Academic Press, 2002, 0366-6352.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.336
Organization unit Faculty of Science
Tags International impact, Reviewed
Changed by Changed by: RNDr. Marta Farková, CSc., učo 546. Changed: 25/2/2013 12:44.
Abstract
This study demonstrates the applicability of Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOFMS) in multicomponent analysis of amino acids with various chemometric methods like experimental design, partial least squares (PLS), and artificial neural networks (ANN). Several negative factors influencing reproducibility of intensities were systematically studied and their influence was eliminated. Binary mixture of L-alanine and L-isoleucine were analyzed and evaluated using PLS and ANN. The results obtained show the possibility to use MALDI-TOFMS in quantitative analysis of amino acids.
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