HAVLIŠ, Jan, Vlastimil DOHNAL, Alma Luísa REVILLA VÁZQUEZ, Libor KOZÁK and Josef HAVEL. Determination of 5-methyl-2'-deoxycytidine 5'-monophosphate and 2'-deoxycytidine 5'-monophosphate in the cell homogenate using HPLC. 14th IMD, Litomyšl, Czech Rep.: n / a, 1999. n / a.
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Basic information
Original name Determination of 5-methyl-2'-deoxycytidine 5'-monophosphate and 2'-deoxycytidine 5'-monophosphate in the cell homogenate using HPLC
Name in Czech Stanovení 5-metyl-2'-deoxycytidin-5'-monofosfáte a 2'-deoxycytidin-5'-monofosfáte v buněčném homogenátu pomocí HPLC
Authors HAVLIŠ, Jan (203 Czech Republic, guarantor), Vlastimil DOHNAL (203 Czech Republic), Alma Luísa REVILLA VÁZQUEZ (484 Mexico), Libor KOZÁK (203 Czech Republic) and Josef HAVEL (203 Czech Republic).
Edition 14th IMD, Litomyšl, Czech Rep. n / a, 1999.
Publisher n / a
Other information
Original language English
Type of outcome Audiovisual works
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/99:00021155
Organization unit Faculty of Science
Keywords in English 5-methylcytosine; HPLC; MALDI TOF MS; ED-ANN; quantification
Tags 5-methylcytosine, ED-ANN, HPLC, MALDI TOF MS, quantification
Changed by Changed by: doc. Mgr. Jan Havliš, Dr., učo 743. Changed: 18/1/2007 14:45.
Abstract
MALDI-TOF MS is a suitable method for extremely fast semi-quantitative determination of purines and pyrimidines in urine. MVA (multivariate approach), represented by experimental design combined with ANN, is a more correct approach to optimization of the separation conditions than that of classical SVA (single variation method) and enables faster optimization of the method with lower number of experiments.
Abstract (in Czech)
MALDI-TOF MS je metodou vhodnou pro velmi rychlé semi-kvantitativní stanovení purinů a pyrimidinů v moči. MVA (multivariační přístup), reprezentovaný kombinací plánování pokusů s ANN, je správnějším přístupem k optimalizaci podmínek separace než klasická SVA (metoda změny jednoho parametru) a umožňuje rychlejší optimalizaci metody nižším počtem nutných experimentů.
Links
MSM 143100011, plan (intention)Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles
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