| HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. Talanta. Elsevier Science, 2002, vol. 56, No 5, p. 905-913. ISSN 0039-9140. |
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@article{483756,
author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
article_number = {5},
keywords = {electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.},
volume = {56},
year = {2002}
}
TY - JOUR
ID - 483756
AU - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY - 2002
TI - Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.
JF - Talanta
VL - 56
IS - 5
SP - 905
EP - 905
PB - Elsevier Science
SN - 00399140
KW - electrochemical impedance spectroscopy
KW - EIS
KW - DNA
KW - bis-intercalator
KW - echinomycin;
N2 - The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.
ER -
HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. \textit{Talanta}. Elsevier Science, 2002, vol.~56, No~5, p.~905-913. ISSN~0039-9140.
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