Electrochemical impedance spectroscopy of polynucleotide
adsorption.

J 2002

Electrochemical impedance spectroscopy of polynucleotide adsorption.

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ a Vladimír VETTERL

Základní údaje

Originální název

Electrochemical impedance spectroscopy of polynucleotide adsorption.

Autoři

STRAŠÁK, Luděk (203 Česká republika), Jakub DVOŘÁK (203 Česká republika), Stanislav HASOŇ (203 Česká republika) a Vladimír VETTERL (203 Česká republika, garant)

Vydání

Bioelectrochemistry, The Netherlands, Elsevier, 2002, 1567-5394

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.463

Kód RIV

RIV/00216224:14310/02:00007362

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly A adsorption; differential capacitance

Štítky

differential capacitance, DNA adsorption, EIS, electrochemical impedance spectroscopy, poly a adsorption, polyadenylic acid adsorption

Změněno: 28. 1. 2003 10:57, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. from the frequency dependence of the impedance of the electrode double layer represented in a complex plane impedance plot (Nyquist plot, Cole-Cole plot), the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded that the desorption of ssDNA (single stranded, denatured) is accompanied by higher dielectric losses than the desorption of dsDNA (double stranded, native). This can be explained by the higher flexibilioty of ssDNA compared to the dsDNA. The capacitance peak of single stranded polyadenylic acid observed at pH 8 around -1.3 V splits at lopw frequencies in two peaks.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ a Vladimír VETTERL. Electrochemical impedance spectroscopy of polynucleotide adsorption. Bioelectrochemistry. The Netherlands: Elsevier, 2002, roč. 56, 1-2, s. 37-41. ISSN 1567-5394.

@article{483762,
   author = {Strašák, Luděk and Dvořák, Jakub and Hasoň, Stanislav and Vetterl, Vladimír},
   article_location = {The Netherlands},
   article_number = {1-2},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly A adsorption; differential capacitance},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Electrochemical impedance spectroscopy of polynucleotide adsorption.},
   volume = {56},
   year = {2002}
}

TY  - JOUR
ID  - 483762
AU  - Strašák, Luděk - Dvořák, Jakub - Hasoň, Stanislav - Vetterl, Vladimír
PY  - 2002
TI  - Electrochemical impedance spectroscopy of polynucleotide adsorption.
JF  - Bioelectrochemistry
VL  - 56
IS  - 1-2
SP  - 37
EP  - 37
PB  - Elsevier
SN  - 15675394
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - polyadenylic acid adsorption
KW  - poly A adsorption
KW  - differential capacitance
N2  - The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. from the frequency dependence of the impedance of the electrode double layer represented in a complex plane impedance plot (Nyquist plot, Cole-Cole plot), the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded that the desorption of ssDNA (single stranded, denatured) is accompanied by higher dielectric losses than the desorption of dsDNA (double stranded, native). This can be explained by the higher flexibilioty of ssDNA compared to the dsDNA. The capacitance peak of single stranded polyadenylic acid observed at pH 8 around -1.3 V splits at lopw frequencies in two peaks.
ER  -

STRAŠÁK, Luděk, Jakub DVOŘÁK, Stanislav HASOŇ a Vladimír VETTERL. Electrochemical impedance spectroscopy of polynucleotide adsorption. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2002, roč.~56, 1-2, s.~37-41. ISSN~1567-5394.

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