Impedance analysis of DNA and DNA-drug interactions on thin
mercury film electrodes.

J 2002

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

HASOŇ, Stanislav; Jakub DVOŘÁK; František JELEN and Vladimír VETTERL

Basic information

Original name

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

Authors

HASOŇ, Stanislav (203 Czech Republic); Jakub DVOŘÁK (203 Czech Republic); František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)

Edition

Critical Reviews in Analytical Chemistry, Boca Raton (Florida, USA), CRC Press, 2002, 1040-8347

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.074

RIV identification code

RIV/00216224:14310/02:00007363

Organization unit

Faculty of Science

Keywords in English

electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;

Abstract

V originále

Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

IAA4004002, research and development project

Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces

Cite

HASOŇ, Stanislav; Jakub DVOŘÁK; František JELEN and Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. Critical Reviews in Analytical Chemistry. Boca Raton (Florida, USA): CRC Press, 2002, vol. 32, No 2, p. 167-179. ISSN 1040-8347.

@article{483763,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   article_location = {Boca Raton (Florida, USA)},
   article_number = {2},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;},
   language = {eng},
   issn = {1040-8347},
   journal = {Critical Reviews in Analytical Chemistry},
   title = {Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.},
   volume = {32},
   year = {2002}
}

TY  - JOUR
ID  - 483763
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2002
TI  - Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
JF  - Critical Reviews in Analytical Chemistry
VL  - 32
IS  - 2
SP  - 167
EP  - 167
PB  - CRC Press
SN  - 10408347
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - echinomycin
KW  - DNA-echinomycin interaction
KW  - mercury film electrodes;
N2  - Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.
ER  -

HASOŇ, Stanislav; Jakub DVOŘÁK; František JELEN and Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. \textit{Critical Reviews in Analytical Chemistry}. Boca Raton (Florida, USA): CRC Press, 2002, vol.~32, No~2, p.~167-179. ISSN~1040-8347.

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