| NOVÁKOVÁ, Soňa, Magdalena TELNAROVÁ a Zdeněk GLATZ. Inhibition study of rhodanese by means of electrophoretically mediated microanalysis. Journal of Chromatography A. Netherlands: Elsevier, 2003, roč. 990, (1+2), s. 189-194. ISSN 0021-9606. |
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@article{484267,
author = {Nováková, Soňa and Telnarová, Magdalena and Glatz, Zdeněk},
article_location = {Netherlands},
article_number = {(1+2)},
keywords = {Capillary electrophoresis; Rhodenase; EMMA; Inhibition},
language = {eng},
issn = {0021-9606},
journal = {Journal of Chromatography A},
title = {Inhibition study of rhodanese by means of electrophoretically mediated microanalysis},
volume = {990},
year = {2003}
}
TY - JOUR
ID - 484267
AU - Nováková, Soňa - Telnarová, Magdalena - Glatz, Zdeněk
PY - 2003
TI - Inhibition study of rhodanese by means of electrophoretically mediated microanalysis
JF - Journal of Chromatography A
VL - 990
IS - (1+2)
SP - 189
EP - 189
PB - Elsevier
SN - 00219606
KW - Capillary electrophoresis
KW - Rhodenase
KW - EMMA
KW - Inhibition
N2 - A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of the bovine liver rhodanese by 2-oxoglutarate. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. The estimated value of KI for 2-oxoglutarate was 3.62 x 10-4 ą 1.43 x 10-4 M with respect to cyanide and 1.40 x 10-3 ą 1.60 x 10-4 M with respect to thiosulfate. Moreover the type of inhibition was also evaluated. The findings of 2-oxogluatarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data.
ER -
NOVÁKOVÁ, Soňa, Magdalena TELNAROVÁ a Zdeněk GLATZ. Inhibition study of rhodanese by means of electrophoretically mediated microanalysis. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2003, roč.~990, (1+2), s.~189-194. ISSN~0021-9606.
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