NEDĚLNÍK, Jan and Jana ŘEPKOVÁ. Genetické modifikace pícních jetelovin (TRANSGENOSIS IN FORAGE LEGUMES). Úroda. Praha, 2002, vol. 2002, No 12, p. 28;29, 2 pp. ISSN 0139-6013.
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Basic information
Original name Genetické modifikace pícních jetelovin
Name (in English) TRANSGENOSIS IN FORAGE LEGUMES
Authors NEDĚLNÍK, Jan (203 Czech Republic) and Jana ŘEPKOVÁ (203 Czech Republic, guarantor).
Edition Úroda, Praha, 2002, 0139-6013.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 40100 4.1 Agriculture, Forestry, and Fisheries
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/02:00007551
Organization unit Faculty of Science
Keywords in English forage legumes; genetic manipulation; resistance genes; stress tolerance; digestibility
Tags digestibility, forage legumes, genetic manipulation, resistance genes, stress tolerance
Changed by Changed by: prof. RNDr. Jana Řepková, CSc., učo 530. Changed: 2/10/2006 13:34.
Abstract
Forage legumes are very important components of animal feed and are capable to supply nitrogen demand from symbiotic associations with Rhizobium. Genetic variability of alfalfa (Medicago sativa), red clover (Trifolium pratense) and white clover (T. repens) appears to be limited. Therefore incorporation of various genes from other species via genetic transformation offers potential. The use of genetically engineered plasmids carried by Agrobacterium tumefaciens is considered an ideal system for forage legumes. The use of A. rhizogenes also offers a convenient technique for genetic manipulation of legumes. Limited transformation success with particle bombardment of intact meristems and pollen has been reported. Most of the currently used transformation methods require regeneration of transformed cells into whole plants from cell suspension or callus tissue culture. Advanced cultivars of most forage legumes have not shown high level of plant regeneration. This low frequency of regeneration may create problems with transformation methods. Molecular engineering is impacting modern agriculture and opens tremendous possibilities. Gene technology has proven to be a powerful tool to help create variability that would not occur with conventional means. The first transgenic alfalfa cultivars will be markered early into the 21st century. They will possess characteristics such as herbicide resistance, insect resistance, enhanced forage yield, improved digestibility and a variety of stress tolerance traits. Output traits such as better protein utilisation and plant vaccines are also being considered.
Abstract (in English)
Forage legumes are very important components of animal feed and are capable to supply nitrogen demand from symbiotic associations with Rhizobium. Genetic variability of alfalfa (Medicago sativa), red clover (Trifolium pratense) and white clover (T. repens) appears to be limited. Therefore incorporation of various genes from other species via genetic transformation offers potential. The use of genetically engineered plasmids carried by Agrobacterium tumefaciens is considered an ideal system for forage legumes. The use of A. rhizogenes also offers a convenient technique for genetic manipulation of legumes. Limited transformation success with particle bombardment of intact meristems and pollen has been reported. Most of the currently used transformation methods require regeneration of transformed cells into whole plants from cell suspension or callus tissue culture. Advanced cultivars of most forage legumes have not shown high level of plant regeneration. This low frequency of regeneration may create problems with transformation methods. Molecular engineering is impacting modern agriculture and opens tremendous possibilities. Gene technology has proven to be a powerful tool to help create variability that would not occur with conventional means. The first transgenic alfalfa cultivars will be markered early into the 21st century. They will possess characteristics such as herbicide resistance, insect resistance, enhanced forage yield, improved digestibility and a variety of stress tolerance traits. Output traits such as better protein utilisation and plant vaccines are also being considered.
Links
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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