J 2003

Precise 3D image alignment in micro-axial tomography

MATULA, Petr, Michal KOZUBEK, Florian STAIER a Michael HAUSMANN

Základní údaje

Originální název

Precise 3D image alignment in micro-axial tomography

Autoři

MATULA, Petr (203 Česká republika, garant), Michal KOZUBEK (203 Česká republika), Florian STAIER (276 Německo) a Michael HAUSMANN (276 Německo)

Vydání

Journal of Microscopy, Oxford, Blackwell Science, 2003, 0022-2720

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.779

Kód RIV

RIV/00216224:14330/03:00008544

Organizační jednotka

Fakulta informatiky

UT WoS

000181298100008

Klíčová slova anglicky

assignment; image fusion; image registration; Kuhn-Munkres algorithm; micro-axial tomography; resolution improvement

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 6. 5. 2009 10:56, doc. RNDr. Petr Matula, Ph.D.

Anotace

V originále

Micro axial tomography is a challenging technique in microscopy which improves quantitative imaging especially in cytogenetic applications by means of defined sample rotation under the microscope objective. The advantage of micro-axial tomography is an effective improvement of the precision of distance measurements between point-like objects. Under certain circumstances, the effective (3D) resolution can be improved by optimized acquisition depending on subsequent, multi-perspective image recording of the same objects followed by reconstruction methods. This requires, however, a very precise alignment of the tilted views. We present a novel feature-based image alignment method with a precision better than the full width at half maximum of the point spread function. The features are the positions (centres of gravity) of all fluorescent objects observed in the images (e.g. cell nuclei, fluorescent signals inside cell nuclei, fluorescent beads, etc.). Thus, real alignment precision depends on the localization precision of these objects. The method automatically determines the corresponding objects in subsequently tilted perspectives using a weighted bipartite graph. The optimum transformation function is computed in a least squares manner based on the coordinates of the centres of gravity of the matched objects. The theoretically feasible precision of the method was calculated using computer-generated data and confirmed by tests on real image series obtained from data sets of 200 nm fluorescent nano-particles. The advantages of the proposed algorithm are its speed and accuracy, which means that if enough objects are included, the real alignment precision is better than the axial localization precision of a single object. The alignment precision can be assessed directly from the algorithm's output. Thus, the method can be applied not only for image alignment and object matching in tilted view series in order to reconstruct (3D) images, but also to validate the experimental performance (e.g. mechanical precision of the tilting). In practice, the key application of the method is an improvement of the effective spatial (3D) resolution, because the well-known spatial anisotropy in light microscopy can be overcome. This allows more precise distance measurements between point-like objects.

Návaznosti

IBS5004010, projekt VaV
Název: Vývoj nových diagnostických technik pro onkologii
Investor: Akademie věd ČR, Vývoj nových diagnostických technik pro onkologii
MSM 143300002, záměr
Název: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii