The four-channel electrochemical immunosensor for detection of
herbicides based on phenoxyalkanoic acids.

J 2002

The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.

KAŠNÁ, Andrea a Petr SKLÁDAL

Základní údaje

Originální název

The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.

Autoři

KAŠNÁ, Andrea (203 Česká republika) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

International Journal of Environmental and Analytical Chemistry, Velká Británie, Taylor & Francis, 2002, 0306-7319

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.765

Kód RIV

RIV/00216224:14310/02:00007720

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Electrochemical immunosensor; Multichannel biosensor; Herbicides; Phenoxyalkanoic acids

Štítky

Electrochemical immunosensor, Herbicides, Multichannel biosensor, Phenoxyalkanoic acids

Změněno: 28. 4. 2003 15:50, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

The electrochemical immunosensor combining three monoclonal antibodies on the same measuring element produced by screen-printing was constructed for the semiquantitative group-specific detection of herbicides belonging to phenoxyalkanoic acids. The combination of different monoclonal antibodies covalently immobilized on three working electrodes and one additional electrode containing immobilized albumin provided a more reliable detection of phenoxyalkanoic acids. Peroxidase-2,4-dichlorophenoxyacetic acid conjugate was used as a tracer suitable for all the antibodies employed in a competitive immunoassay. Several herbicides were used to characterize the immunosensor. The developed immunosensor was tested on samples of surface water spiked with different herbicides and binary mixtures of herbicides. The proposed approach seems feasible for rapid screening of large series of samples for the presence of herbicides.

Návaznosti

GA525/97/0924, projekt VaV

Název: Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě

Investor: Grantová agentura ČR, Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě

Citovat

KAŠNÁ, Andrea a Petr SKLÁDAL. The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids. International Journal of Environmental and Analytical Chemistry. Velká Británie: Taylor & Francis, 2002, roč. 83, č. 2, s. 101-109. ISSN 0306-7319.

@article{486235,
   author = {Kašná, Andrea and Skládal, Petr},
   article_location = {Velká Británie},
   article_number = {2},
   keywords = {Electrochemical immunosensor; Multichannel biosensor; Herbicides; Phenoxyalkanoic acids},
   language = {eng},
   issn = {0306-7319},
   journal = {International Journal of Environmental and Analytical Chemistry},
   title = {The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.},
   volume = {83},
   year = {2002}
}

TY  - JOUR
ID  - 486235
AU  - Kašná, Andrea - Skládal, Petr
PY  - 2002
TI  - The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.
JF  - International Journal of Environmental and Analytical Chemistry
VL  - 83
IS  - 2
SP  - 101-109
EP  - 101-109
PB  - Taylor & Francis
SN  - 03067319
KW  - Electrochemical immunosensor
KW  - Multichannel biosensor
KW  - Herbicides
KW  - Phenoxyalkanoic acids
N2  - The electrochemical immunosensor combining three monoclonal antibodies on the same measuring element produced by screen-printing was constructed for the semiquantitative group-specific detection of herbicides belonging to phenoxyalkanoic acids. The combination of different monoclonal antibodies covalently immobilized on three working electrodes and one additional electrode containing immobilized albumin provided a more reliable detection of phenoxyalkanoic acids. Peroxidase-2,4-dichlorophenoxyacetic acid conjugate was used as a tracer suitable for all the antibodies employed in a competitive immunoassay. Several herbicides were used to characterize the immunosensor. The developed immunosensor was tested on samples of surface water spiked with different herbicides and binary mixtures of herbicides. The proposed approach seems feasible for rapid screening of large series of samples for the presence of herbicides.
ER  -

KAŠNÁ, Andrea a Petr SKLÁDAL. The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids. \textit{International Journal of Environmental and Analytical Chemistry}. Velká Británie: Taylor \&{} Francis, 2002, roč.~83, č.~2, s.~101-109. ISSN~0306-7319.

Podrobný výpis o publikaci