2003
Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology
KUTHAN, Martin, Frederic DEVAUX, Blanka JANDEROVA, Iva SLANINOVA, Claude JACQ et. al.Základní údaje
Originální název
Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology
Název česky
Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology
Autoři
KUTHAN, Martin (703 Slovensko), Frederic DEVAUX (250 Francie), Blanka JANDEROVA (203 Česká republika), Iva SLANINOVA (203 Česká republika, garant), Claude JACQ (250 Francie) a Zdena PALKOVA (203 Česká republika)
Vydání
Molecular Microbiology, Blackwell Publishing Ltd, 2003, 0950-382X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 5.563
Kód RIV
RIV/00216224:14330/03:00011319
Organizační jednotka
Fakulta informatiky
UT WoS
000180776700011
Klíčová slova anglicky
Saccharomyces; colony morphology; yeast;extracellular matrix; DNA microarrays
Změněno: 22. 6. 2009 12:12, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell-cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty-transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions.
Česky
Although colonies from Saccharomyces cerevisiae laboratory strains are smooth, those isolated from nature exhibit a structured fluffy pattern. Environmental scanning electron microscopy shows that the cells within wild fluffy colonies are connected by extracellular matrix (ECM) material. This material contains a protein of about 200 kDa unrelated to the flocculins, proteins involved in cell-cell adhesion in liquid media. The matrix material binds to concanavalin A. Within a few passages on rich agar medium, the wild strains switch from the fluffy to the smooth colony morphology. This domestication is accompanied by loss of the ECM and by extensive changes in gene expression as detected by DNA microarrays. The expression of about 320 genes was changed in smooth colonies. The major changes comprise carbohydrate metabolism, cell wall, water channels, Ty-transposons and subtelomeric genes, iron homeostasis, vitamin metabolism and cell cycle and polarity. The growth in fluffy colonies may represent a metabolic strategy for survival of yeast under unfavourable conditions that is switched off under felicitous laboratory conditions.
Návaznosti
| GA204/02/0650, projekt VaV |
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