Development in vitro of the neuromusculature of two strigeid
trematodes, Apatemon cobitidis proterorhini and Cotylurus
erraticus

J 2003

Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus

STEWART, Michael T., Angela MOUSLEY, Božena KOUBKOVÁ, Šárka ŠEBELOVÁ, Nikki J. MARKS et. al.

Základní údaje

Originální název

Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus

Autoři

STEWART, Michael T. (826 Velká Británie a Severní Irsko, garant), Angela MOUSLEY (826 Velká Británie a Severní Irsko), Božena KOUBKOVÁ (826 Velká Británie a Severní Irsko), Šárka ŠEBELOVÁ (203 Česká republika), Nikki J. MARKS (826 Velká Británie a Severní Irsko) a David Wiliam HALTON (826 Velká Británie a Severní Irsko)

Vydání

International Journal for Parasitology, Australia, Elsevier Science Ltd. 2003, 0020-7519

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.881

Kód RIV

RIV/00216224:14310/03:00008017

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000182644100010

Klíčová slova anglicky

Strigeid trematodes; Apatemon; Cotylurus; In vitro cultivation; Neuromusculature; Reproductive system; Confocal microscopy

Štítky

Apatemon, confocal microscopy, Cotylurus, In vitro cultivation, Neuromusculature, Reproductive system, Strigeid trematodes

Změněno: 20. 5. 2003 13:58, RNDr. Božena Koubková, Ph.D.

Anotace

V originále

Confocal microscopy interfaced with cytochemical procedures has been used to monitor development of the major muscle systems and associated serotoninergic (5-HT, 5-hydroxytryptamine) and peptidergic (FaRP, FMRFamide-related peptide) innervation of the strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus during cultivation in vitro. Sexually undifferentiated metacercariae were successfully grown to ovigerous adults using tissue culture medium NCTC 135, chicken serum and egg albumen. Eggs were produced after 5 days in culture but had abnormal shells and failed to embryonate. 5-HT and FaRP (the flatworm FaRP, GYIRFamide) were localised immunocytochemically in both central and peripheral nervous systems of developing worms. During cultivation, the central serotoninergic and FaRPergic neuronal pathways of the forebody became more extensive, but retained the same basic orthogonal arrangement as found in the excysted metacercaria. Longitudinal extensor and flexor muscles of the hindbody provide support for the developing reproductive complex. The male reproductive tracts were established in advance (day 3) of those of the female system (day 4); completion of the latter was marked by the appearance of the ootype/egg chamber. The inner longitudinal muscle fibres of the female tract appeared prior to the outer and more densely arranged circular muscles. Circular fibres dominate the muscle complement of both alimentary and reproductive tracts. 5-HT- and GYIRFamide-immunoreactivities were demonstrable in the central nervous system (CNS) and subtegumental parasympathetic nervous system (PNS) throughout the culture period, but innervation of the developing reproductive structures was reactive just for 5-HT. Only at the onset of egg production was FaRP-IR observed in the reproductive system and was expressed only in the innervation of the ootype, a finding consistent with the view that FaRPs may regulate egg assembly in platyhelminths.

Návaznosti

GA524/00/0844, projekt VaV

Název: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Grantová agentura ČR, Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Citovat

STEWART, Michael T., Angela MOUSLEY, Božena KOUBKOVÁ, Šárka ŠEBELOVÁ, Nikki J. MARKS a David Wiliam HALTON. Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus. International Journal for Parasitology. Australia: Elsevier Science Ltd., 2003, roč. 33, č. 4, s. 413-424. ISSN 0020-7519.

@article{486369,
   author = {Stewart, Michael T. and Mousley, Angela and Koubková, Božena and Šebelová, Šárka and Marks, Nikki J. and Halton, David Wiliam},
   article_location = {Australia},
   article_number = {4},
   keywords = {Strigeid trematodes; Apatemon; Cotylurus; In vitro cultivation; Neuromusculature; Reproductive system; Confocal microscopy},
   language = {eng},
   issn = {0020-7519},
   journal = {International Journal for Parasitology},
   title = {Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus},
   volume = {33},
   year = {2003}
}

TY  - JOUR
ID  - 486369
AU  - Stewart, Michael T. - Mousley, Angela - Koubková, Božena - Šebelová, Šárka - Marks, Nikki J. - Halton, David Wiliam
PY  - 2003
TI  - Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus
JF  - International Journal for Parasitology
VL  - 33
IS  - 4
SP  - 413-424
EP  - 413-424
PB  - Elsevier Science Ltd.
SN  - 00207519
KW  - Strigeid trematodes
KW  - Apatemon
KW  - Cotylurus
KW  - In vitro cultivation
KW  - Neuromusculature
KW  - Reproductive system
KW  - Confocal microscopy
N2  - Confocal microscopy interfaced with cytochemical procedures has been used to monitor development of the major muscle systems and associated serotoninergic (5-HT, 5-hydroxytryptamine) and peptidergic (FaRP, FMRFamide-related peptide) innervation of the strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus during cultivation in vitro. Sexually undifferentiated metacercariae were successfully grown to ovigerous adults using tissue culture medium NCTC 135, chicken serum and egg albumen. Eggs were produced after 5 days in culture but had abnormal shells and failed to embryonate. 5-HT and FaRP (the flatworm FaRP, GYIRFamide) were localised immunocytochemically in both central and peripheral nervous systems of developing worms. During cultivation, the central serotoninergic and FaRPergic neuronal pathways of the forebody became more extensive, but retained the same basic orthogonal arrangement as found in the excysted metacercaria. Longitudinal extensor and flexor muscles of the hindbody provide support for the developing reproductive complex. The male reproductive tracts were established in advance (day 3) of those of the female system (day 4); completion of the latter was marked by the appearance of the ootype/egg chamber. The inner longitudinal muscle fibres of the female tract appeared prior to the outer and more densely arranged circular muscles. Circular fibres dominate the muscle complement of both alimentary and reproductive tracts. 5-HT- and GYIRFamide-immunoreactivities were demonstrable in the central nervous system (CNS) and subtegumental parasympathetic nervous system (PNS) throughout the culture period, but innervation of the developing reproductive structures was reactive just for 5-HT. Only at the onset of egg production was FaRP-IR observed in the reproductive system and was expressed only in the innervation of the ootype, a finding consistent with the view that FaRPs may regulate egg assembly in platyhelminths.
ER  -

STEWART, Michael T., Angela MOUSLEY, Božena KOUBKOVÁ, Šárka ŠEBELOVÁ, Nikki J. MARKS a David Wiliam HALTON. Development in vitro of the neuromusculature of two strigeid trematodes, Apatemon cobitidis proterorhini and Cotylurus erraticus. \textit{International Journal for Parasitology}. Australia: Elsevier Science Ltd., 2003, roč.~33, č.~4, s.~413-424. ISSN~0020-7519.

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