Specific determination of cysteine in human urine by capillary
micellar electrokinetic chromatography

J 2003

Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ

Základní údaje

Originální název

Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography

Autoři

ŠEVČÍKOVÁ, Petra (203 Česká republika) a Zdeněk GLATZ (203 Česká republika, garant)

Vydání

Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2003, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008072

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000183660100015

Klíčová slova anglicky

urine; cysteine; MEKC; on-column reaction

Štítky

cysteine, MEKC, On-column reaction, urine

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.

Návaznosti

GA203/03/1125, projekt VaV

Název: Využití kapilární zónové elektroforézy pro studium enzymů

Investor: Grantová agentura ČR, Využití kapilární zónové elektroforézy pro studium enzymů

Citovat

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2003, roč. 26, č. 8, s. 734-738. ISSN 1615-9306.

@article{487342,
   author = {Ševčíková, Petra and Glatz, Zdeněk},
   article_location = {Weinheim},
   article_number = {8},
   keywords = {urine; cysteine; MEKC; on-column reaction},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography},
   volume = {26},
   year = {2003}
}

TY  - JOUR
ID  - 487342
AU  - Ševčíková, Petra - Glatz, Zdeněk
PY  - 2003
TI  - Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography
JF  - Journal of Separation Science
VL  - 26
IS  - 8
SP  - 734-738
EP  - 734-738
PB  - WILEY-VCH Verlag GmbH & Co.
SN  - 16159306
KW  - urine
KW  - cysteine
KW  - MEKC
KW  - on-column reaction
N2  - This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.
ER  -

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2003, roč.~26, č.~8, s.~734-738. ISSN~1615-9306.

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