Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA

TRNKOVÁ, Libuše, René KIZEK and Jan VACEK. Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA. In XVII th International Symposium on Bioelectrochemictry and Bioenergetics. 1st ed. Florence, Italy: The Bioelectrochemical Society, 2003, p. 139.

Basic information

• Original name: Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
• Authors: TRNKOVÁ, Libuše (203 Czech Republic, guarantor), René KIZEK (203 Czech Republic) and Jan VACEK (203 Czech Republic).
• Edition: 1. vyd. Florence, Italy, XVII th International Symposium on Bioelectrochemictry and Bioenergetics, p. 139-139, 2003.
• Publisher: The Bioelectrochemical Society

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10405 Electrochemistry
• Country of publisher: Italy
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/03:00008112
• Organization unit: Faculty of Science
• Keywords in English: azidothymidine; hanging mercury drop electrode; square wave voltammetry; elimination voltammetry with linear scan; oligonucleotides; chromosomal DNA
• Tags: azidothymidine, chromosomal DNA, hanging mercury drop electrode, Oligonucleotides, square wave voltammetry

Abstract

Azidothymidine ( AZT, Zidovudine) is a synthetic nucleoside analogue with activity against human immunodeficiency virus type 1 (HIV). Recently, our working group has developed and optimised a square wave voltammetric method for analysis of AZT in biological samples. The aim of the study is the determination of AZT in the presence of synthetic oligonucleotides (ODNs) and calf thymus ssDNA or dsDNA on the hanging mercury drop electrode. Both AZT and DNA yield voltammetric signals on the mercury electrode; AZT provides an irreversible reduction peak at the potential near -1.1 V (vs. Ag/AgCl/3M KCl) and DNA provides a reduction peak of adenine and cytosine at the potential ca. -1.4 V. Square wave voltammetric signals of AZT and DNA were measured in phosphate buffer (pH 8.0) at a frequency of 2000 Hz and accumulation time of 10 s. We found that AZT peak height is not affected by the presence of DNA and therefore we assume that electron transfer proceeds through the adsorption layer of ssDNA and/or dsDNA. The detection limit of AZT is 1 nM and 250 nM in absence and in presence of ssDNA (10 mg/mL), respectively. The SWV is an effective tool for the AZT determination incorporated into oligonucleotide chains. This promising methodology ca be used to study AZT cytotoxicity and kinetics of AZT metabolism in HIV positive patients.

Links

• GA203/02/0422, research and development project: Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry

• IAA1163201, research and development project: Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.