ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK, Jiří LENFELD, Jana PRODĚLALOVÁ, Jana SUČIKOVÁ and Soňa ŠTRUMCOVÁ. Immunomagnetic separation of Salmonella cells using newly designed carriers. Journal of Chromatography A. Amsterdam: Elsevier, 2003, vol. 1009, 1-2, p. 215-221. ISSN 0021-9673. |
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@article{488220, author = {Španová, Alena and Rittich, Bohuslav and Horák, Daniel and Lenfeld, Jiří and Prodělalová, Jana and Sučiková, Jana and Štrumcová, Soňa}, article_location = {Amsterdam}, article_number = {1-2}, keywords = {Salmonella spp. magnetic sorbents ; immobilization; affinity sorbents; hydroxyethyl methacrylate; glycidyl methacrylate; ethylene methacrylate; proteinase K; enzymes; antibodies}, language = {eng}, issn = {0021-9673}, journal = {Journal of Chromatography A}, title = {Immunomagnetic separation of Salmonella cells using newly designed carriers}, volume = {1009}, year = {2003} }
TY - JOUR ID - 488220 AU - Španová, Alena - Rittich, Bohuslav - Horák, Daniel - Lenfeld, Jiří - Prodělalová, Jana - Sučiková, Jana - Štrumcová, Soňa PY - 2003 TI - Immunomagnetic separation of Salmonella cells using newly designed carriers JF - Journal of Chromatography A VL - 1009 IS - 1-2 SP - 215-221 EP - 215-221 PB - Elsevier SN - 00219673 KW - Salmonella spp. magnetic sorbents KW - immobilization KW - affinity sorbents KW - hydroxyethyl methacrylate KW - glycidyl methacrylate KW - ethylene methacrylate KW - proteinase K KW - enzymes KW - antibodies N2 - Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells. ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK, Jiří LENFELD, Jana PRODĚLALOVÁ, Jana SUČIKOVÁ and Soňa ŠTRUMCOVÁ. Immunomagnetic separation of Salmonella cells using newly designed carriers. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2003, vol.~1009, 1-2, p.~215-221. ISSN~0021-9673.
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