ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK, Jiří LENFELD, Jana PRODĚLALOVÁ, Jana SUČIKOVÁ and Soňa ŠTRUMCOVÁ. Immunomagnetic separation of Salmonella cells using newly designed carriers. Journal of Chromatography A. Amsterdam: Elsevier, 2003, vol. 1009, 1-2, p. 215-221. ISSN 0021-9673.
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Basic information
Original name Immunomagnetic separation of Salmonella cells using newly designed carriers
Authors ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Bohuslav RITTICH (203 Czech Republic), Daniel HORÁK (203 Czech Republic), Jiří LENFELD (203 Czech Republic), Jana PRODĚLALOVÁ (203 Czech Republic), Jana SUČIKOVÁ (203 Czech Republic) and Soňa ŠTRUMCOVÁ (203 Czech Republic).
Edition Journal of Chromatography A, Amsterdam, Elsevier, 2003, 0021-9673.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.922
RIV identification code RIV/00216224:14310/03:00008145
Organization unit Faculty of Science
Keywords in English Salmonella spp. magnetic sorbents ; immobilization; affinity sorbents; hydroxyethyl methacrylate; glycidyl methacrylate; ethylene methacrylate; proteinase K; enzymes; antibodies
Tags affinity sorbents, antibodies, enzymes, ethylene methacrylate, glycidyl methacrylate, hydroxyethyl methacrylate, immobilization, proteinase K, Salmonella spp. magnetic sorbents
Tags Reviewed
Changed by Changed by: doc. Ing. Bohuslav Rittich, CSc., učo 1639. Changed: 23/11/2006 08:47.
Abstract
Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells.
Links
GA203/00/1339, research and development projectName: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích
Investor: Czech Science Foundation, Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application
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