Development of a disposable electrochemical immunosensor for
detection of the herbicide acetochlor

J 2003

Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

SOLNÁ, Renáta, Petr SKLÁDAL a Sergei EREMIN

Základní údaje

Originální název

Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Autoři

SOLNÁ, Renáta (203 Česká republika), Petr SKLÁDAL (203 Česká republika, garant) a Sergei EREMIN (643 Rusko)

Vydání

International Journal of Environmental and Analytical Chemistry, Velká Británie, Taylor & Francis, 2003, 0306-7319

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.616

Kód RIV

RIV/00216224:14310/03:00008148

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000184128300008

Klíčová slova anglicky

Electrochemical immunosensor;Screen-printed sensor;Acetochlor

Štítky

Acetochlor, Electrochemical immunosensor, Screen-printed sensor

Změněno: 5. 9. 2003 14:03, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

The electrochemical immunosensor for the detection of the herbicide acetochlor was developed. Initially, the feasibility of the immunosensor was verified using the standard ELISA technique employing the competitive assay format with immobilized acetochlor. The assay principle of the disposable immunochemical biosensor was similar. The screen-printed electrode system served as the transducer, acetochlor was covalently immobilized on the surface of the gold working electrode activated with a self-assembled monolayer of cystamine. Acetochlor was linked to this layer through S-acetylmercaptosuccinic anhydride (AMSA). A limited amount of anti-acetochlor polyclonal rabbit antibody in solution competed with the analyzed acetochlor and the secondary goat anti-rabbit antibody labeled with peroxidase was chosen for detection. The amperometric measurement of peroxidase activity was carried out using 5?aminosalicylic acid (ASA) and hydrogen peroxide as substrates. Successful regeneration of the sensing surface was achieved using pepsin at pH 2 when analyzing samples of water. However, deterioration of the sensing surface in the presence of food samples (corn, carrot, potato and milk) required adopting the disposable assay format. The detection limits of the immunosensor were 25 mg/l (drinking water), 60 mg/l (surface water) and 5 mg/l (milk), thus the sensitivity of the immunosensor assay was not sufficient for drinking water analysis. At present, the developed immunosensor allows only qualitative detection of acetochlor above the maximum residual levels in food. The potential repeated use of the immunosensor remains an important issue for future optimization.

Návaznosti

GA525/97/0924, projekt VaV

Název: Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě

Investor: Grantová agentura ČR, Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě

Citovat

SOLNÁ, Renáta, Petr SKLÁDAL a Sergei EREMIN. Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor. International Journal of Environmental and Analytical Chemistry. Velká Británie: Taylor & Francis, 2003, roč. 83, 7-8, s. 609-620. ISSN 0306-7319.

@article{488274,
   author = {Solná, Renáta and Skládal, Petr and Eremin, Sergei},
   article_location = {Velká Británie},
   article_number = {7-8},
   keywords = {Electrochemical immunosensor;Screen-printed sensor;Acetochlor},
   language = {eng},
   issn = {0306-7319},
   journal = {International Journal of Environmental and Analytical Chemistry},
   title = {Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor},
   volume = {83},
   year = {2003}
}

TY  - JOUR
ID  - 488274
AU  - Solná, Renáta - Skládal, Petr - Eremin, Sergei
PY  - 2003
TI  - Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor
JF  - International Journal of Environmental and Analytical Chemistry
VL  - 83
IS  - 7-8
SP  - 609-620
EP  - 609-620
PB  - Taylor & Francis
SN  - 03067319
KW  - Electrochemical immunosensor;Screen-printed sensor;Acetochlor
N2  - The electrochemical immunosensor for the detection of the herbicide acetochlor was developed. Initially, the feasibility of the immunosensor was verified using the standard ELISA technique employing the competitive assay format with immobilized acetochlor. The assay principle of the disposable immunochemical biosensor was similar. The screen-printed electrode system served as the transducer, acetochlor was covalently immobilized on the surface of the gold working electrode activated with a self-assembled monolayer of cystamine. Acetochlor was linked to this layer through S-acetylmercaptosuccinic anhydride (AMSA). A limited amount of anti-acetochlor polyclonal rabbit antibody in solution competed with the analyzed acetochlor and the secondary goat anti-rabbit antibody labeled with peroxidase was chosen for detection. The amperometric measurement of peroxidase activity was carried out using 5?aminosalicylic acid (ASA) and hydrogen peroxide as substrates. Successful regeneration of the sensing surface was achieved using pepsin at pH 2 when analyzing samples of water. However, deterioration of the sensing surface in the presence of food samples (corn, carrot, potato and milk) required adopting the disposable assay format. The detection limits of the immunosensor were 25 mg/l (drinking water), 60 mg/l (surface water) and 5 mg/l (milk), thus the sensitivity of the immunosensor assay was not sufficient for drinking water analysis. At present, the developed immunosensor allows only qualitative detection of acetochlor above the maximum residual levels in food. The potential repeated use of the immunosensor remains an important issue for future optimization.
ER  -

SOLNÁ, Renáta, Petr SKLÁDAL a Sergei EREMIN. Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor. \textit{International Journal of Environmental and Analytical Chemistry}. Velká Británie: Taylor \&{} Francis, 2003, roč.~83, 7-8, s.~609-620. ISSN~0306-7319.

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