J 2003

Screen-printed multienzyme arrays for use in amperometric batch and flow systems

SAPELNIKOVA, Svetlana; Eva DOCK; Renáta SOLNÁ; Petr SKLÁDAL; Tautgirdas RUZGAS et. al.

Basic information

Original name

Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Authors

SAPELNIKOVA, Svetlana (643 Russian Federation); Eva DOCK (752 Sweden); Renáta SOLNÁ (203 Czech Republic); Petr SKLÁDAL (203 Czech Republic, guarantor); Tautgirdas RUZGAS (440 Lithuania) and Jenny EMNEUS (752 Sweden)

Edition

Analytical and Bioanalytical Chemistry, Heidelberg, Springer, 2003, 1618-2642

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.715

RIV identification code

RIV/00216224:14310/03:00008149

Organization unit

Faculty of Science

UT WoS

000185155600019

Keywords in English

Screen-printed sensor; Cholinesterase; Enzyme electrode
Changed: 5/9/2003 14:23, prof. RNDr. Petr Skládal, CSc.

Abstract

In the original language

The screen-printed technology of electrode fabrication allows construction of amperometric devices suitable for the combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor-arrays in the batch mode as well as in the flow-injection system are discussed.

Links

GA525/97/0924, research and development project
Name: Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě
Investor: Czech Science Foundation, Trace analysis of toxic PCB and pesticides using detection and separation methods based on antibody specifity