Piezoelectric quartz crystal sensors applied for bioanalytical
assays and characterization of affinity interactions

J 2003

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

SKLÁDAL, Petr

Základní údaje

Originální název

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Autoři

SKLÁDAL, Petr (203 Česká republika, garant)

Vydání

Journal of the Brazilian Chemical Society, Sao Paulo, Sociedade Brasileira de Química, 2003, 0103-5053

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Brazílie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.895

Kód RIV

RIV/00216224:14310/03:00008863

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000184902600002

Klíčová slova anglicky

Piezoelectric biosensor; Review

Štítky

Piezoelectric biosensor, Review

Změněno: 5. 9. 2003 14:31, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

Návaznosti

OC 518.30, projekt VaV

Název: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Citovat

SKLÁDAL, Petr. Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions. Journal of the Brazilian Chemical Society. Sao Paulo: Sociedade Brasileira de Química, 2003, roč. 14, č. 4, s. 491-502. ISSN 0103-5053.

@article{488278,
   author = {Skládal, Petr},
   article_location = {Sao Paulo},
   article_number = {4},
   keywords = {Piezoelectric biosensor; Review},
   language = {eng},
   issn = {0103-5053},
   journal = {Journal of the Brazilian Chemical Society},
   title = {Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions},
   volume = {14},
   year = {2003}
}

TY  - JOUR
ID  - 488278
AU  - Skládal, Petr
PY  - 2003
TI  - Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
JF  - Journal of the Brazilian Chemical Society
VL  - 14
IS  - 4
SP  - 491-502
EP  - 491-502
PB  - Sociedade Brasileira de Química
SN  - 01035053
KW  - Piezoelectric biosensor
KW  - Review
N2  - This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.
ER  -

SKLÁDAL, Petr. Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions. \textit{Journal of the Brazilian Chemical Society}. Sao Paulo: Sociedade Brasileira de Química, 2003, roč.~14, č.~4, s.~491-502. ISSN~0103-5053.

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