2003
Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
SKLÁDAL, PetrZákladní údaje
Originální název
Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions
Autoři
SKLÁDAL, Petr (203 Česká republika, garant)
Vydání
Journal of the Brazilian Chemical Society, Sao Paulo, Sociedade Brasileira de Química, 2003, 0103-5053
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Brazílie
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.895
Kód RIV
RIV/00216224:14310/03:00008863
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000184902600002
Klíčová slova anglicky
Piezoelectric biosensor; Review
Štítky
Změněno: 5. 9. 2003 14:31, prof. RNDr. Petr Skládal, CSc.
Anotace
V originále
This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.
Návaznosti
OC 518.30, projekt VaV |
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