Aleuria aurantia lectin displays novel binding mode for fucose.
Crystal structure and ligand interaction of a new family of
fucolectins.

D 2003

Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins.

WIMMEROVÁ, Michaela, Edward MITCHELL, Jean-Frederic SANCHEZ, Catherine GAUTIER, Hugues LORTAT-JACOB et. al.

Základní údaje

Originální název

Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins.

Autoři

WIMMEROVÁ, Michaela (203 Česká republika, garant), Edward MITCHELL (826 Velká Británie a Severní Irsko), Jean-Frederic SANCHEZ (250 Francie), Catherine GAUTIER (250 Francie), Hugues LORTAT-JACOB (250 Francie), Nechama GILBOA-GARBER (376 Izrael) a Anne IMBERTY (250 Francie)

Vydání

Grenoble, France, 12th European Carbohydrate Symposium, s. 93-93, 2003

Nakladatel

CERMAV-CNRS

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008864

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

aleuria aurantia; lectin; crystal structure; fungi

Štítky

aleuria aurantia, Crystal Structure, fungi, lectin

Příznaky

Mezinárodní význam

Změněno: 15. 11. 2006 11:39, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

Aleuria aurantia lectin (AAL) is a fungal protein that specifically recognised fucose moiety in glycans. The crystal structure of AAL complexed with fucose has been solved at 1.5 A resolution. AAL displays a dimer composition, each subunit consists of a six-bladed beta-propeller fold and of a small antiparallel two-strands beta-sheet that play a role in dimerization. Primary sequence of AAL contains six internal repeats [1] corresponded to particular blades, crystal structure reveals five fucose molecules per AAL monomer in binding sites formed by two adjacent blades. Surface plasmon resonance experiments have been performed on a series of fucose containing oligosaccharides to determine equilibrium dissociation constants for lectine-oligosaccharide interaction. Measurements confirm the broad specificity of the lectin, with a slight preference for alpha-Fuc1-2Gal disaccharide. AAL shows high sequence similarity with a lectin from plant pathogenic bacterium Ralstonia solanacearum (RSL) [2]. As RSL is smaller (91 amino acid residues compared to 312 of AAL), only two typical internal repeats can be found in sequence. SPR studies demonstrated that RSL shares similar specificity as AAL but highly prefers alpha-Fuc1-2 linkage. [1] Fukumori, F., Takeuchi, N., Hagiwara, T., Ohbayashi, H., Endo, T., Kochibe, N., Nagata, Y., and Kobata, A J. Biochem. 107, 190-196 (1990) [2] Sudakevitz, D., Imberty, A., and Gilboa-Garber, N., J. Biochem. 132, 353-358 (2002)

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

WIMMEROVÁ, Michaela, Edward MITCHELL, Jean-Frederic SANCHEZ, Catherine GAUTIER, Hugues LORTAT-JACOB, Nechama GILBOA-GARBER a Anne IMBERTY. Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins. In 12th European Carbohydrate Symposium. Grenoble, France: CERMAV-CNRS, 2003, s. 93.

@inproceedings{488300,
   author = {Wimmerová, Michaela and Mitchell, Edward and Sanchez, JeanandFrederic and Gautier, Catherine and LortatandJacob, Hugues and GilboaandGarber, Nechama and Imberty, Anne},
   address = {Grenoble, France},
   booktitle = {12th European Carbohydrate Symposium},
   keywords = {aleuria aurantia; lectin; crystal structure; fungi},
   language = {eng},
   location = {Grenoble, France},
   pages = {93-93},
   publisher = {CERMAV-CNRS},
   title = {Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins.},
   year = {2003}
}

TY  - JOUR
ID  - 488300
AU  - Wimmerová, Michaela - Mitchell, Edward - Sanchez, Jean-Frederic - Gautier, Catherine - Lortat-Jacob, Hugues - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2003
TI  - Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins.
PB  - CERMAV-CNRS
CY  - Grenoble, France
KW  - aleuria aurantia
KW  - lectin
KW  - crystal structure
KW  - fungi
N2  - Aleuria aurantia lectin (AAL) is a fungal protein that specifically recognised fucose moiety in glycans. The crystal structure of AAL complexed with fucose has been solved at 1.5 A resolution. AAL displays a dimer composition, each subunit consists of a six-bladed beta-propeller fold and of a small antiparallel two-strands beta-sheet that play a role in dimerization. Primary sequence of AAL contains six internal repeats [1] corresponded to particular blades, crystal structure reveals five fucose molecules per AAL monomer in binding sites formed by two adjacent blades. Surface plasmon resonance experiments have been performed on a series of fucose containing oligosaccharides to determine equilibrium dissociation constants for lectine-oligosaccharide interaction. Measurements confirm the broad specificity of the lectin, with a slight preference for alpha-Fuc1-2Gal disaccharide. AAL shows high sequence similarity with a lectin from plant pathogenic bacterium Ralstonia solanacearum (RSL) [2]. As RSL is smaller (91 amino acid residues compared to 312 of AAL), only two typical internal repeats can be found in sequence. SPR studies demonstrated that RSL shares similar specificity as AAL but highly prefers alpha-Fuc1-2 linkage. [1] Fukumori, F., Takeuchi, N., Hagiwara, T., Ohbayashi, H., Endo, T., Kochibe, N., Nagata, Y., and Kobata, A J. Biochem. 107, 190-196 (1990) [2] Sudakevitz, D., Imberty, A., and Gilboa-Garber, N., J. Biochem. 132, 353-358 (2002)
ER  -

WIMMEROVÁ, Michaela, Edward MITCHELL, Jean-Frederic SANCHEZ, Catherine GAUTIER, Hugues LORTAT-JACOB, Nechama GILBOA-GARBER a Anne IMBERTY. Aleuria aurantia lectin displays novel binding mode for fucose. Crystal structure and ligand interaction of a new family of fucolectins. In \textit{12th European Carbohydrate Symposium}. Grenoble, France: CERMAV-CNRS, 2003, s.~93.

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