Specificity and kinetics of oligosaccharide recognition by RSL,
a fucose-binding lectin from the plant pathogen Ralstonia
solanacearum.

D 2003

Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB et. al.

Základní údaje

Originální název

Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.

Autoři

WIMMEROVÁ, Michaela (203 Česká republika, garant), Anne IMBERTY (250 Francie), Dvora SUDAKEVITZ (376 Izrael), Gerard CHAMBAT (250 Francie), Hugues LORTAT-JACOB (250 Francie) a Nechama GILBOA-GARBER (376 Izrael)

Vydání

Bangalore, Indie, XVII International Symposium on Glycoconjugates, s. 23-23, 2003

Nakladatel

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Indie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008866

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Ralstonia solanacearum; lectin; surface plasmon resonance

Štítky

lectin, ralstonia solanacearum, surface plasmon resonance

Příznaky

Mezinárodní význam

Změněno: 15. 11. 2006 11:39, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

Ralstonia solanacearum is a worldwide distributed plant aggressive pathogen which causes lethal wilt in many crops. Its extracts contain a fucose-binding lectin that has been recently purified and characterized [1]. Its 90 amino acid sequence contains two repeating domains, with strong similarity to the fucose-binding lectin of the mushroom Aleuria aurantia (AAL), which is also a soil inhabitant. Surface plasmon resonance experiments demonstrate that the lectin binds strongly to fragments of fucose-containing xyloglucan polysaccharide purified from plant cell walls. This binding can diversely be inhibited by fucose and fucose-containing oligosaccharides. Best inhibition was obtained with oligosaccharides containing an aFuc(1-2)Gal terminal disaccharide, particularly XG9 (Glc4 Xyl3 Gal Fuc) which is a structurally well determined plant oligosaccharide that has previously been demonstrated to have biological activity in plants [2]. Our results are in agreement with the assumption that RSL plays a role in binding of the bacterium to specific oligosaccharides that are present in the primary root hairs cell wall of the host plant.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB a Nechama GILBOA-GARBER. Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum. In XVII International Symposium on Glycoconjugates. Bangalore, Indie: AS, 2003, s. 23.

@inproceedings{488305,
   author = {Wimmerová, Michaela and Imberty, Anne and Sudakevitz, Dvora and Chambat, Gerard and LortatandJacob, Hugues and GilboaandGarber, Nechama},
   address = {Bangalore, Indie},
   booktitle = {XVII International Symposium on Glycoconjugates},
   keywords = {Ralstonia solanacearum; lectin; surface plasmon resonance},
   language = {eng},
   location = {Bangalore, Indie},
   pages = {23-23},
   publisher = {AS},
   title = {Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.},
   year = {2003}
}

TY  - JOUR
ID  - 488305
AU  - Wimmerová, Michaela - Imberty, Anne - Sudakevitz, Dvora - Chambat, Gerard - Lortat-Jacob, Hugues - Gilboa-Garber, Nechama
PY  - 2003
TI  - Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum.
PB  - AS
CY  - Bangalore, Indie
KW  - Ralstonia solanacearum
KW  - lectin
KW  - surface plasmon resonance
N2  - Ralstonia solanacearum is a worldwide distributed plant aggressive pathogen which causes lethal wilt in many crops. Its extracts contain a fucose-binding lectin that has been recently purified and characterized [1]. Its 90 amino acid sequence contains two repeating domains, with strong similarity to the fucose-binding lectin of the mushroom Aleuria aurantia (AAL), which is also a soil inhabitant. Surface plasmon resonance experiments demonstrate that the lectin binds strongly to fragments of fucose-containing xyloglucan polysaccharide purified from plant cell walls. This binding can diversely be inhibited by fucose and fucose-containing oligosaccharides. Best inhibition was obtained with oligosaccharides containing an aFuc(1-2)Gal terminal disaccharide, particularly XG9 (Glc4 Xyl3 Gal Fuc) which is a structurally well determined plant oligosaccharide that has previously been demonstrated to have biological activity in plants [2]. Our results are in agreement with the assumption that RSL plays a role in binding of the bacterium to specific oligosaccharides that are present in the primary root hairs cell wall of the host plant.
ER  -

WIMMEROVÁ, Michaela, Anne IMBERTY, Dvora SUDAKEVITZ, Gerard CHAMBAT, Hugues LORTAT-JACOB a Nechama GILBOA-GARBER. Specificity and kinetics of oligosaccharide recognition by RSL, a fucose-binding lectin from the plant pathogen Ralstonia solanacearum. In \textit{XVII International Symposium on Glycoconjugates}. Bangalore, Indie: AS, 2003, s.~23.

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